Promega Corporation

TMB Stabilized Substrate for Horseradish Peroxidase

TMB Stabilized Substrate is a stable, ready-to-use TMB (3,3´, 5,5´-tetramethylbenzidine) color development substrate for localization of horseradish peroxidase-conjugated antibodies on dot blots and Western blots. It is easier to use than 4-chloro-1-naphthol (CN), which must be prepared immediately before use. TMB Stabilized Substrate comes premixed and fully diluted in a proprietary buffer containing less than 0.5% organic solvent.

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TMB Stabilized Substrate for Horseradish Peroxidase

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TMB Stabilized Substrate for Horseradish Peroxidase

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  • Helix Cabinet

This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.


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200ml
- W4121 R$ 416,00 Add to cart


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Storage Conditions

Store at 22–25°C.

For product intended use please see Patents & Disclaimers tab.

Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells.Figure 1. Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells.

The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of total protein (µg). Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, HRP Conjugate secondary antibody (Cat.# W4021) used at 1:2,500; blot imaged with TMB Stabilized Substrate for Horseradish Peroxidase (Cat.# W4121).

Use Restrictions

W4121 For Research Use Only. Not for Use in Diagnostic Procedures.

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