Western Blue® Stabilized Substrate for Alkaline Phosphatase is a stable, ready-to-use substrate for Western blots and immunoscreening. It is a mixture of 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and nitro blue tetrazolium (NBT) in a proprietary stabilizing buffer. Western Blue® Substrate should be used directly and without dilution. This liquid substrate deposits a permanent dark purple stain on membrane sites bearing alkaline phosphatase. Western Blue® Substrate is as sensitive as other reagents based on the BCIP/NBT formulation.
Expand to Read More »
« Collapse Content
Western Blue® Stabilized Substrate for Alkaline Phosphatase
This product can be added to a
This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.
Already a Helix Customer?
Store at room temperature, 22–25°C.
For product intended use please see Patents & Disclaimers tab.
The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of β-actin (ng). Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, AP Conjugate secondary antibody (Cat.# S3721) used at 1:2,500; blot imaged with Western® Blue Stabilized Substrate for Alkaline Phosphatase (Cat.# S3841).
S3841 For Research Use Only. Not for Use in Diagnostic Procedures.
Your country is set to Brazil. Your language is set to Português. Please select the language that will best suit your needs:
This is correct, continue to site »
I need additional help