Promega Corporation

GSH/GSSG-Glo™ Assay

The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells. A change in GSH levels is important in the assessment of toxicological responses and is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay provides a simple, rapid multiwell-plate format where stable luminescent signals are correlated with either the total GSH or the GSSG concentration of a sample directly in culture wells. Both total glutathione and GSSG determinations are based on the reaction where GSH-d...

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GSH/GSSG-Glo™ Assay

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GSH/GSSG-Glo™ Assay

 Components

  • Passive Lysis Buffer, 5X

    E194B1 x 1ml
  • DTT

    P117B1 x 250μl
  • Reconstitution Buffer with esterase

    V144A1 x 10ml
  • Glutathione Reaction Buffer

    V163A1 x 10ml
  • NEM, 25mM

    V164A1 x 50μl
  • Luciferin-NT

    V687A1 x 100μl
  • Glutathione S-Transferase

    V689B1 x 500μl
  • Glutathione, 5mM

    V690A1 x 100μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10ml
- V6611 R$ 1.947,00 Add to cart

GSH/GSSG-Glo™ Assay

Close Window

GSH/GSSG-Glo™ Assay

 Components

  • Passive Lysis Buffer, 5X

    E194C1 x 5ml
  • DTT

    P117C1 x 1.25ml
  • Reconstitution Buffer with esterase

    V144B1 x 50ml
  • Glutathione Reaction Buffer

    V163B1 x 50ml
  • NEM, 25mM

    V164B1 x 250μl
  • Luciferin-NT

    V687B1 x 500μl
  • Glutathione S-Transferase

    V689B3 x 500μl
  • Glutathione, 5mM

    V690A2 x 100μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


50ml
- V6612 R$ 7.792,00 Add to cart


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Storage Conditions

Store at –20°C protected from light.

For product intended use please see Patents & Disclaimers tab.

GSH/GSSG ratio determination in different cell lines.Figure 1. GSH/GSSG ratio determination in different cell lines.

Cells were treated for 60 minutes with 40µM menadione or vehicle (0.1% DMSO). The protocol for adherent cells was performed and the ratio calculated as directed in TM344. Other cell lines tested: K562, CHO and Jurkat cells.

GSH-dependent conversion of a GSH probe, Luciferin-NT, to luciferin.Figure 2. GSH-dependent conversion of a GSH probe, Luciferin-NT, to luciferin by a glutathione S-transferase enzyme is coupled to a firefly luciferase reaction.

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Workflow for the GSH/GSSG-Glo Assay.Figure 3. Workflow for the GSH/GSSG-Glo™ Assay.
Limit of detection using reduced and oxidized glutathione.Figure 4. Limit of detection using reduced and oxidized glutathione.

GSH and GSSG standards were assayed in a 96-well plate using the GSH/GSSG-Glo™ System and a GloMax® Luminometer. The results were averaged and plotted on the graphs above. A linear trend line was generated and correlation coefficients calculated for the fit of the data to the trend line. Limits of detection were 2.5nM GSH and 0.5nM GSSG.

Use Restrictions

V6611, V6612 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V6611, V6612 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

V6611, V6612 Certain applications of this product may require licenses from others.

V6611, V6612 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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