CellTiter 96® AQ_ueous One Solution Cell Proliferation Assay (MTS)

The CellTiter 96® AQ_ueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. The CellTiter 96® AQ_ueous One Solution Reagent contains a tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] and an electron coupling reagent (phenazine ethosulfate; PES). PES has enhanced chemical stability, which allows it to be combined with MTS to form a stable solution. The CellTiter 96® AQ_ueous Assay uses phenazine methosulfate (PMS) as the ele...

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The CellTiter 96® AQ_ueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. The CellTiter 96® AQ_ueous One Solution Reagent contains a tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] and an electron coupling reagent (phenazine ethosulfate; PES). PES has enhanced chemical stability, which allows it to be combined with MTS to form a stable solution. The CellTiter 96® AQ_ueous Assay uses phenazine methosulfate (PMS) as the electron coupling reagent, and PMS Solution and MTS Solution are supplied separately. PES has enhanced chemical stability, which allows it to be combined with MTS to form a stable solution.

Assays are performed by adding a small amount of the CellTiter 96® AQ_ueous One Solution Reagent directly to culture wells, incubating for 1–4 hours and then recording absorbance at 490nm with a 96-well plate reader. The quantity of formazan product as measured by the amount of 490nm absorbance is directly proportional to the number of living cells in culture.

If you currently use a [³H]-thymidine incorporation assay, addition of the CellTiter 96® AQ_ueous One Solution Reagent can be substituted for the pulse of [³H]-thymidine at the time point in the assay when the pulse of radioactive thymidine is usually added. Previous bioassay data comparing [³H]-thymidine incorporation to the MTS-based CellTiter 96® AQ_ueous Assay and the original MTT-based CellTiter 96® Assay demonstrate that tetrazolium reagents can be substituted for [³H]-thymidine incorporation.

Features - Benefits

• Simplify Colorimetric Viability Assays: "Add-incubate-measure" format (single-step reagent addition) enables design of homogeneous high-throughput screening assays.
• Use a Single Solution: Use as a single solution, filter sterilized and ready to add to assay plates (unlike MTT).
• Perform Fewer Steps: Perform the assay in 96-well plates with no washing or cell harvesting. Also eliminates solubilization steps normally required for MTT assays.
• Gain Flexibility: Plates can be read and returned to incubator for further color development (unlike MTT).
• Avoid Organic Solvents: Requires no volatile organic solvent to solubilize the formazan product (unlike MTT).
• Non-Radioactive: Requires no scintillation cocktail or radioactive waste disposal (unlike [³H]-thymidine incorporation assays).
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

• Cell proliferation.
• Cell viability.
• Cytotoxicity.
• Chemosensitivity.
• Cell attachment determination.
• Chemotaxis.

For more information, see the Protocols & Applications Guide.

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