CellTiter-Glo® One Solution Assay

The CellTiter-Glo® One Solution Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which indicates the presence of metabolically active cells. This frozen, ready-to-use format is based on the original CellTiter-Glo® Luminescence Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when preparing reagent. The CellTiter-Glo® Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) in 96- to 1536-well format, and cell prolife...

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The CellTiter-Glo® One Solution Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which indicates the presence of metabolically active cells. This frozen, ready-to-use format is based on the original CellTiter-Glo® Luminescence Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when preparing reagent. The CellTiter-Glo® Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) in 96- to 1536-well format, and cell proliferation and cytotoxicity assays.

The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® One Solution Assay generates a stable "glow-type" luminescent signal with a half-life of greater than three hours. This extended half-life eliminates the need for reagent injectors and provides flexibility for continuous or batch-mode processing of multiple plates.

Features - Benefits

• Convenient: No reagent preparation is required; simply thaw and "add-mix-measure". Volumes convenient for HTS applications.
• Homogeneous: "Add-mix-measure" format reduces the number of plate-handling steps.
• Fast: Data can be recorded 10 minutes after reagent addition.
• Sensitive: Measures cells at numbers below the detection limits of standard colorimetric and fluorometric assays.
• Flexible: Can be used with various multiwell formats (96-, regular or low-volume 384- and 1536-well plates). Data can be recorded by luminometer or CCD camera imaging device.
• Robust: Stable luminescent signal with a half-life >3 hours, depending on cell type and culture medium used.
• Ability to Multiplex: Can be used with other nonlytic compatible assay chemistries from Promega.

Applications

• Cell viability.
• Cytotoxicity.
• Cell proliferation.
• High-throughput screening.

Notes

Cat.# G8461 is sufficient for 1,000 assays at 100µl/assay in 96-well plates or 4,000 assays at 25µl/assay in 384-well plates.
Cat.# G8462 is sufficient for 5,000 assays at 100µl/assay in 96-well plates or 20,000 assays at 25µl/assay in 384-well plates.

References

1. Farfan, A. et al. (2004) Multiplexing homogeneous cell-based assays. Cell Notes 10, 2–5.
2. Riss, T., Moravec, R. and Niles, A. (2005) Selecting cell-based assays for drug discovery screening. Cell Notes 13, 16-21.

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