ReliaPrep™ 96 gDNA Miniprep HT System

The ReliaPrep™ 96 gDNA Miniprep HT System provides a simple and reliable method for the rapid isolation of gDNA in a multiwell format. gDNA may be purified from blood and Oragene®•Discover sample collection devices. The purified gDNA can be used directly in PCR assays, microarrays and next-generation sequencing applications. The use of paramagnetic particles for DNA capture eliminates the need for centrifugation or vacuum manifolds, making the system suitable for full automation. In addition, the system does not require an organic solvent, making it safe and convenient. DNA yields of...

Expand to Read More »

The ReliaPrep™ 96 gDNA Miniprep HT System provides a simple and reliable method for the rapid isolation of gDNA in a multiwell format. gDNA may be purified from blood and Oragene®•Discover sample collection devices. The purified gDNA can be used directly in PCR assays, microarrays and next-generation sequencing applications. The use of paramagnetic particles for DNA capture eliminates the need for centrifugation or vacuum manifolds, making the system suitable for full automation. In addition, the system does not require an organic solvent, making it safe and convenient. DNA yields of up to 12µg are expected from input blood volumes of 350µl, depending on the WBC count of the sample. Saliva samples can have variable amounts of gDNA, and up to 18µg or more of DNA may be recovered from a 700µl Oragene® collection device sample.

Features - Benefits

• Improve Productivity: Walkaway automation of genomic DNA extraction.
• Eliminate Sample Rework: Robust, precipitation-free protocol, no chance of "lost pellets".
• Simplify Workflow: High yields of pure DNA from pristine and challenged or hemolysed samples.
• Reduce Time to Results: Pure gDNA ready for demanding applications—samples in solution—no resuspension required.

Applications

Purified DNA is suitable for common assays including genotyping, PCR, qPCR and sequencing.

« Collapse Content