Wizard® Plus SV Minipreps DNA Purification Systems

Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time Wizard® Plus SV Minipreps Start-Up Kits are available. Purchase a Wizard® Plus SV Minipreps DNA Purification System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, please contact your Promega representative or an authorized Promega distributor.

The Wizard® Plus SV Minipreps DNA Purification System, a silica membrane-based system, provides a simple and reliable method for r...

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Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time Wizard® Plus SV Minipreps Start-Up Kits are available. Purchase a Wizard® Plus SV Minipreps DNA Purification System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, please contact your Promega representative or an authorized Promega distributor.

The Wizard® Plus SV Minipreps DNA Purification System, a silica membrane-based system, provides a simple and reliable method for rapid isolation of plasmid DNA. The entire miniprep procedure can be completed in 45 minutes or less, depending on the number of samples processed. Using the system, plasmid DNA can be purified from 1–10ml of overnight E. coli culture. The purified plasmid DNA can be used directly for automated fluorescent BigDye® terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation. It also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).

Features - Benefits

• Improved Productivity: 20 minipreps processed in less than 45 minutes.
• High Performance: 1–20μg of high-quality plasmid DNA, enough for multiple applications.
• Safety and Convenience: No phenol extractions or precipitations required.
• Flexibility: Choice of spin (microcentrifuge) or vacuum purification formats.
• Consistent Quality: Alkaline protease step improves plasmid quality.
• Confidence in Results: Purified DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector in BigDye® terminator sequencing.

Applications

For more information, see the Protocols & Applications Guide.

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