PolyATtract® System 1000

The PolyATtract® System 1000 isolates messenger RNA directly from crude cell or tissue lysates, eliminating the need for total RNA isolations. This system uses the MagneSphere® technology for the purification of poly(A)+ RNA, eliminating the need for oligo(dT) cellulose columns. The increased yield of mRNA using this system allows the detection of low-copy-number mRNAs in relatively small amounts of material using Northern blot analysis. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation, cDNA synthesis, PCR analysis, ribonuclease (RNase) protection assays, primer ...

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The PolyATtract® System 1000 isolates messenger RNA directly from crude cell or tissue lysates, eliminating the need for total RNA isolations. This system uses the MagneSphere® technology for the purification of poly(A)+ RNA, eliminating the need for oligo(dT) cellulose columns. The increased yield of mRNA using this system allows the detection of low-copy-number mRNAs in relatively small amounts of material using Northern blot analysis. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation, cDNA synthesis, PCR analysis, ribonuclease (RNase) protection assays, primer extension and Northern blots.

The MagneSphere® Technology Magnetic Separation Stands can be used in conjunction with any of the PolyATtract® Systems and are ideal for applications requiring multiple paramagnetic isolations of biomolecules.

Features - Benefits

• Improved Productivity: mRNA purification directly from tissue or cells in 45 minutes or less. Allows quick collection of magnetic particles.
• Flexibility: Works with tissue amounts from 5mg–2g per isolation. Magnetic separation stand (Cat.# Z5410) accommodates 1.5ml, 2ml, 15ml and 50ml tube sizes.
• Convenience: No lengthy ethanol precipitation steps, phenol:chloroform extractions, or overnight ultracentrifugation through cesium chloride gradients and lithium chloride (LiCl) precipitations.

Applications

For more information, see the Protocols & Applications Guide.

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