Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time SV Total RNA Isolation Start-Up Kits are available. Purchase an SV Total RNA Isolation System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, please contact your Promega representative or an authorized Promega distributor.
The SV Total RNA Isolation System provides a fast and simple technique for preparation of intact total RNA from tissues, cultured cells and white blood ...
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The SV Total RNA Isolation System provides a fast and simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour. Using this membrane-based purification system, up to 60mg of tissue can be processed per purification, depending on tissue type. The system incorporates a DNase treatment step directly on the minicolumn membrane. This step substantially reduces genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, and there is no DNase carryover in the final RNA preparation.
Sample types processed using the SV Total RNA Isolation System.
For more information, see the Protocols & Applications Guide.
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SV Total RNA Isolation System
RNA Lysis Buffer (RLA)
RNA Dilution Buffer (RDA)
RNA Wash Solution (RWA)
DNase Stop Solution (DSA)
Yellow Core Buffer
DNase I (lyophilized)
This product can be added to a
This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.
Already a Helix Customer?
DNase Stop Solution
SV Total RNA Isolation Start-Up Kit, 110V Electrical (250 preps, manifold and free vacuum pump)
Welch Vacuum Pump for North America Electrical
Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity
SV Total RNA Isolation Start-Up Kit, 220V Electrical (250 preps, manifold and free vacuum pump)
Welch Vacuum Pump for Continental Europe Electrical
SV Total RNA Isolation Start-Up Kit, 230V Electrical (250 preps, manifold and free vacuum pump)
Welch Vacuum Pump for Japan Electrical
Red Blood Cell Lysis Solution (CLB)
Store at 22–25°C.
For product intended use please see Patents & Disclaimers tab.
Z3101, Z3100, Z3105, Z3380, Z3382, Z3384, Z3141, Z3051 For Research Use Only. Not for Use in Diagnostic Procedures.
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