Promega Corporation

Protease-Glo™ Assay

The Protease-Glo™ Assay is a novel method to detect and measure protease activities using a genetically engineered firefly (Photinus pyralis) luciferase and represents one example of the GloSensor™ platform technology. The assay uses a circularly permuted firefly luciferase, the GloSensor™-10F protein, with a protease recognition site as the protease substrate. This assay system allows rapid generation of protease substrates through molecular cloning and coupled transcription/translation cell-free expression, thus enabling the facile evaluation of protease function. Oligonucleotides encoding a protease recognition...

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  • Prices valid for customers of Promega Brazil only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Protease-Glo™ Assay

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Protease-Glo™ Assay

 Components

  • 2X Rapid Ligation Buffer

    C671A1 x 200μl
  • Oligo Annealing Buffer

    C838A1 x 1ml
  • Bright-Glo™ Luciferase Assay Substrate

    E263A1 x 1 vial
  • Bright-Glo™ Luciferase Assay Buffer

    E264A1 x 10ml
  • pGloSensor™-10F Linear Vector

    G946A1 x 20μl
  • pGloSensor™-10F(TEV) Control Plasmid

    G947A1 x 100μl
  • TnT® SP6 High-Yield Wheat Germ Master Mix

    L326A1 x 300μl
  • T4 DNA Ligase

    M180A1 x 100u
  • DTT

    P117B1 x 250μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
  • ProTEV Buffer, 20X

    V602A1 x 1ml
  • ProTEV Plus

    V610A1 x 1,000u
1 each
- G9451 R$ 4.061,00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

pGloSensor™-10F Linear Vector

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pGloSensor™-10F Linear Vector

 Components

  • pGloSensor™-10F Linear Vector

    G946A1 x 20μl
1μg
- G9461 R$ 2.436,00 Add to cart

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GenBank®/EMBL Accession Number

pGloSensor™ -10F Linear Vector, EU693492; pGloSensor™ -10F Linear Vector Sequence (.txt file)
pGloSensor™ -10F[TEV] Control Plasmid, EU693493; pGloSensor™ -10F[TEV] Control Plasmid Sequence (.txt file)

Storage Conditions

Store all components at –20°C, except the TnT® SP6 High-Yield Wheat Germ Master Mix, which must be stored at –70°C.

For product intended use please see Patents & Disclaimers tab.

Overview of the Protease-Glo Assay protocol.Figure 1. Overview of the Protease-Glo™ Assay protocol.

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Modulation of firefly luciferase with a polypeptide linker between the N- and C-termini.Figure 2. Modulation of firefly luciferase with a polypeptide linker between the N- and C-termini.

Panel A. New N- and C-termini were created to generate the GloSensor™ protein. Panel B. Insertion of a short polypeptide linker containing a protease recognition sequence greatly reduces luciferase activity. More details are available in the protocol.

Use Restrictions

G9451, G9461 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G9451, G9461 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
NOT FOR USE IN LIVE CELLS OR LIVE ANIMALS—For use in live cells or animals contact Promega regarding the availability of licenses. Researchers may use this product and its derivatives in their own research solely for the extracellular (in vitro) determination of protease activity. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the circularly permuted luciferase reporter of the product and derivatives except that researchers may add oligonucleotides at the site corresponding to the free ends of the linearized product. In addition, researchers must either: (1) use reagents purchased from Promega for all (a) determinations of luminescence activity, (b) extracellular transcription of DNA sequences, and (c) extracellular translation of transcripts; or (2) contact Promega to obtain a license for the use of the product and its derivatives in conjunction with luminescent assay reagents not purchased from Promega. Researchers may transfer derivatives to others provided that recipients agree to be bound by the conditions of this limited use statement prior to such transfer. No other use or transfer of this product or derivatives is authorized without the prior express, written consent of Promega. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

G9451, G9461 U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

G9461 U.S. Pat. No. 5,670,356.

G9451, G9461 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

G9451, G9461 Licensed under U.S. Pat. No. 5,910,628.

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