Faster process. Flexible protocols. Unbeatable results.
A fast and flexible 21-plex for casework, paternity and database testing, the PowerPlex® 21 System provides:
- Rapid thermal cycling, saving time with every run
- Unbeatable inhibitor tolerance, enabling analysis of challenging casework samples
- Optimized protocols for many sample types, including direct amplification from FTA® cards, as well as pretreated non FTA® card punches or swabs
Figure 1. Configuration of the PowerPlex® 21 System. The PowerPlex® 21 System contains all of the 13 CODIS loci: D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX and vWA, plus Amelogenin, Penta D, Penta E, D1S1656, D2S1338, D6S1043, D12S391 and D19S433.
Figure 2. The PowerPlex® 21 System. A single-source template DNA (0.5ng) was amplified using the PowerPlex® 21 System and analyzed with an Applied Biosystems 3130 Genetic Analyzer using a 3kV, 5-second injection. Results were analyzed using GeneMapper® ID software, version 3.2. Panel A. The fluorescein-labeled loci. Panel B. The JOE-labeled loci. Panel C. The TMR-ET-labeled loci. Panel D. The CXR-ET-labeled loci. Panel E. The CC5 Internal Lane Standard 500.
Unbeatable Inhibitor Tolerance
The PowerPlex® 21 System is more tolerant of PCR inhibitors than other STR systems including the PowerPlex® 16 HS System. This allows more interpretable data and less need for re-amplification of samples previously deemed "difficult" due to the presence of inhibitors.
Figure 3. Human genomic DNA (500pg; n = 3) was amplified with the PowerPlex ® 16 HS and 21 Systems in the presence of common inhibitors such as hematin, humic acid and tannic acid. Percent of alleles called are indicated.
Flexibility for Most Sample Types
The PowerPlex® 21 System is designed to work with a variety of sample types, including difficult casework samples and is compatible with direct amplification from FTA® card punches as well as pretreated swabs and Bode Buccal Collector™ device or S&S 903 punches.
Figure 4. Upper Left (4a): Amplification of 500pg of human genomic DNA using the recommended protocol. Amplified products were separated on an Applied Biosystems 3130 Genetic Analyzer (3kV, 5-second injection). Upper Right (4b): Direct amplification of one 1.2mm punch from blood on an FTA card using recommended protocol. Amplified products were separated on Applied Biosystems 3130 Genetic Analyzer (3kV, 5-second injection). Bottom Left (4c): A 1.2mm punch from buccal samples on S&S 903 was pre-treated with prototype PunchSolution™ reagent, then amplified using the recommended protocol. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection). Bottom Right (4d): A swab from buccal samples was pre-treated with SwabSolution™ reagent, then amplified using the recommended protocol. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection).