Maxwell® 16 Clinical System
Maxwell® 16 Clinical System
Maxwell® 16 System
Maxwell® 16 Instruments
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Personal Automation™ for the Clinical Lab
New Clinical Applications for Maxwell® 16 Instrument
Automated DNA Extraction from Buccal Swabs and FFPE samples
Maxwell® 16 Buccal Swab LEV DNA Purification Kit, AS1295
The Maxwell® 16 Buccal Swab LEV DNA Purification Kit provides automated, high DNA yield.
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Improve your patient experience: Use non-invasive buccal swab collection devices
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Reduce repeats and cost: Sufficient yield and purity from a single swab to perform downstream assays
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Fast and easy: Minimal sample transfer step
Promega Quantification Data from One and Two Swabs
| |
ng/µl |
260/280 |
260/230 |
|
|
ng/µl |
260/280 |
260/230 |
| 2 swabs |
121.85 |
1.81 |
1.52 |
|
1 swab |
97.98 |
1.88 |
1.94 |
| 2 swabs |
209.01 |
1.82 |
1.64 |
|
1 swab |
190.01 |
1.73 |
1.4 |
| 2 swabs |
276.45 |
1.87 |
1.74 |
|
1 swab |
65.06 |
1.82 |
1.67 |
| 2 swabs |
274.13 |
1.8 |
1.55 |
|
1 swab |
194.52 |
1.76 |
1.41 |
| 2 swabs |
122.16 |
1.8 |
1.58 |
|
1 swab |
251.36 |
1.96 |
2.09 |
| 2 swabs |
173.5 |
1.91 |
1.82 |
|
1 swab |
121.88 |
1.88 |
1.78 |
| 2 swabs |
235.9 |
1.81 |
1.69 |
|
1 swab |
136.33 |
1.88 |
1.96 |
| AVERAGE |
201.86 |
1.83 |
1.65 |
|
1 swab |
147.6 |
1.91 |
2.15 |
|
|
|
|
|
AVERAGE |
150.59 |
1.85 |
1.8 |
Data suggest DNA concentration is compatible with multiple HLA assays without the need to concentrate. While two swabs did yield more DNA, the amount was not linear. Variability between samples is normal as the amount of DNA recovered will depend on donor and collection technique. Concentration requirements for the SSP kit tested are 75-125ng/µl.
Maxwell® 16 FFPE Plus LEV DNA Purification Kit, AS1135
The improved Maxwell® 16 FFPE Plus LEV DNA Purification Kit provides high yield with shorter processing time.
- High Yield: Sufficient DNA yield to run downstream assays
- Flexible and Fast: Options for 1 hour or overnight digests
- Safe: No xylene or other hazardous solvents for deparaffinization
| Sample |
AS1135 Overnight (ng/µl) |
AS1135 One Hour (ng/µl) |
| Mouse Spleen |
109.9 |
72.35 |
| Mouse Kidney |
122.19 |
122.23 |
| Mouse Brain |
71.26 |
98.3 |
The Maxwell® 16 FFPE Plus LEV DNA Purification Kit is designed to extract high yields of DNA from FFPE tissue samples, with a protocol that allows for the option of a 1 hour proteinase-K incubation (for minimizing processing time), or an overnight incubation (for maximum yield).