Promega Corporation

Real-Time PCR Technologies


This presentation of real-time polymerase chain reaction (PCR) theory and practice is ideal for nonscientists working in the field of Molecular Biology/Biotechnology or for scientists new to real-time PCR techniques who want to understand the fundamentals and gain laboratory experience.

In English: May 26-28, Dec 17-19
In French: April 23-25, Nov 12-14
Additional dates can be arranged by contacting PETAL with a custom training request.

Daily schedule:
9:30 to 17:30 (21 hr)

1400 € VAT excluded for researchers and technicians
1200 € VAT excluded for students

Who should attend:
This practical course is for scientists, teachers, laboratory technicians, researchers and others who would like to learn more about real-time PCR, analysis of results, optimizing reactions and troubleshooting problems.

Maximum number of attendees: 12
A certificate of completion is given to participants.

Course Content
This class is a three-day overview of real-time PCR technologies. Course topics include; brief overview of nucleic acid isolation, comparison between end-point and quantitative PCR (qPCR), review of existing qPCR techniques, setting up a SYBR® Green qPCR and multiplexing PCR.

Lectures Laboratory
Nucleic acid isolation fundamentals Isolate and analyze nucleic acids for use in downstream reactions
Comparative review end-point PCR and real-time PCR techniques  
Review of qPCR techniques  
SYBR® Green PCR: Discuss primer design, optimization, data analysis and application to everyday experiments Use SYBR® dye to track amplification in real–time PCR
Multiplexing PCR: Learn how researchers amplify multiple gene products in a single reaction Fluorescent detection of multiple, amplified gene products

Specific course objectives may vary depending on individual and group interests. Please contact us if you have questions.

Register Now!


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