Promega Corporation

Sample ID and Mixed Sample Detection

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STR Systems

The PowerPlex® 21, 18D and 16 HS Systems offer the power of discrimination of 21, 18 and 16 loci to:

  • Confirm cell or tissue origin and identity
  • Detect cell mixtures or contaminants
  • Overview
  • PowerPlex® 21 System
  • PowerPlex® 18D System
  • PowerPlex® 16 HS System

Cell/Tissue Identification and Characterization

Compare a test sample to a known reference sample(s) to confirm the identity of the test sample or identify key differences between samples.

10620TA

Mixed Sample Analysis

Determine the purity of a sample, identify contaminants or determine the genotype of each cell type in a mixed population.

10621TA

Applications

  • Track and confirm tissue specimen provenance and cell lineage.
  • Detect contaminants, including extraneous tissue or mixed cell populations.
  • Test twin zygosity to determine whether twins are identical or fraternal.
  • Examine genetic changes in cancer development and progression research.
  • Determine fetal gender early in pregnancy.

The PowerPlex® 21 System allows co-amplification and detection of 21 loci, including D1S1656, D2S1338, D3S1358, D5S818, D6S1043, D7S820, D8S1179, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, Amelogenin, CSF1PO, FGA, Penta D, Penta E, TH01, TPOX and vWA. The PowerPlex® 21 System provides all materials necessary to amplify these loci, including a hot-start thermostable DNA polymerase.

Order Powerplex21
10189TA

Advantages of the PowerPlex® 21 System include:

  • Amplifying 21 loci for the highest level of discrimination with extra loci commonly used in Chinese population and complete data coverage with most existing systems.
  • Rapid PCR technology, reducing thermal cycling times to approximately 90 minutes.
  • Improved inhibitor tolerance, making the system more sensitive and robust than other STR amplification systems.
  • Flexible sample input, compatible with direct-amplification from FTA® cards, quick protocol for swabs and other punch cards.
  • A convenient 5X master mix that includes a hot-start thermostable DNA polymerase.

The PowerPlex® 18D System allows co-amplification and detection of 18 loci, including D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, Amelogenin, vWA, D8S1179, TPOX, FGA, D19S433 and D2S1338. The system provides all materials necessary to amplify these loci, including a hot-start thermostable DNA polymerase.

Order Powerplex18D
Configuration of the PowerPlex 18D System.

Advantages of the PowerPlex® 18D System include:

  • Amplifying 18 loci for a higher level of discrimination.
  • Rapid PCR technology, reduced amplification time to less than 1.5 hours.
  • Simplified and shortened sample processing with direct amplification from FTA® cards.
  • A convenient 5X master mix that includes a hot-start thermostable DNA polymerase.

The PowerPlex® 16 HS System allows co-amplification and detection of 16 loci, including Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. The system provides all materials necessary to amplify these loci, including hot-start Taq DNA polymerase.

Order Powerplex16 HS
10603MA_600px

Advantages of the PowerPlex® 16 HS System include:

  • Amplifying 16 loci for a great level of discrimination with confident performance in the presence of inhitiors.
  • Great Sensitivity: Each lot is quality tested to produce full profiles from 100pg of DNA.
  • A convenient 5X master mix that includes hot-start Taq DNA polymerase.

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