High-efficiency protein expression from any gene under the control of a T7 promoter and with a ribosome binding site. Now in convenient 50µl single-use aliquots.
Single Step KRX Competent Cells
Efficient transformation and tightly controlled protein expression. Ideal for rapidly generating new clones for recombinant protein expression.
Regulated Mammalian Expression System
Allows high levels of controlled induction with low basal protein expression, and gives you the convenience of a rapid and sensitive on/off switch for protein expression.
FastBreak™ Cell Lysis Reagent
FastBreak™ Cell Lysis Reagent is designed for the efficient, gentle lysis of E. coli cultures without the need for centrifugation or mechanical cell disruption. The reagent is provided as a 10X concentrate. Add the reagent directly to E. coli cultures. Following a brief incubation, the cells are disrupted, and the protein of interest is released.
In this article we examined the effect of induction time and media on recombinant protein yield and activity in Single Step (KRX) Competent Cells.
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