Promega Corporation

TnT® T7 Insect Cell Extract Protein Expression System

The TnT® T7 Insect Cell Extract Protein Expression System is a convenient, quick, single-tube, coupled transcription/translation system for the cell-free expression of proteins. Protein synthesis reactions are initiated by the addition of a DNA template, eliminating the need for the time-consuming process of in vitro RNA synthesis.

The extract is made from the commonly used Spodoptera frugiperda Sf21 cell line. All components necessary for the transcription/translation are present in the TnT® T7 ICE Master Mix. To initiate protein synthesis, the only component that must be added is the DNA template. Reactions a...

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TnT® T7 Insect Cell Extract Protein Expression System

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TnT® T7 Insect Cell Extract Protein Expression System

 Components

  • Luciferase ICE T7 Control DNA

    L105B1 x 10μg
  • TnT® T7 ICE Master Mix

    L110A1 x 400μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
10 reactions
- L1101 R$ 690,00 Add to cart

TnT® T7 Insect Cell Extract Protein Expression System

Close Window

TnT® T7 Insect Cell Extract Protein Expression System

 Components

  • Luciferase ICE T7 Control DNA

    L105B1 x 10μg
  • TnT® T7 ICE Master Mix

    L110A4 x 400μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
40 reactions
- L1102 R$ 2.411,00 Add to cart

pF25A ICE T7 Flexi® Vector

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pF25A ICE T7 Flexi® Vector

 Components

  • pF25A ICE T7 Flexi® Vector

    L106A1 x 20μg
20μg
- L1061 R$ 1.034,00 Add to cart

pF25K ICE T7 Flexi® Vector

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pF25K ICE T7 Flexi® Vector

 Components

  • pF25K ICE T7 Flexi® Vector

    L108A1 x 20μg
20μg
- L1081 R$ 1.034,00 Add to cart


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GenBank®/EMBL Accession Number

pF25A ICE T7 Flexi® Vector Accession Number EU754721; pF25A ICE T7 Flexi® Vector Vector sequence text file.
pF25K ICE T7 Flexi® Vector Accession Number  EU754722; pF25K ICE T7 Flexi® Vector Vector sequence text file.

Storage Conditions

Store at –70°C.

For product intended use please see Patents & Disclaimers tab.

9695MD_500Figure 1. Representation of the Protein Expression System.

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Typical protein yields using TNT T7 Insect Cell Extract Protein Expression System.Figure 2. Typical protein yields using the TnT® T7 Insect Cell Extract Protein Expression System.

Use Restrictions

L1101, L1102, L1061, L1081 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

L1061, L1081 European Pat. No. 1685247 and other patents pending.

L1061, L1081 Patent Pending.

L1101, L1102, L1061, L1081 Ezure, T., Suzuki, T., Higashide, S., Shintani, E., Endo, K., Kobayashi, S., Shikata, M., Ito, M., Tanimizu, K., Nishimura, O. (2006) Cell-free protein synthesis system prepared from insect cells by freeze-thawing. Biotechnol. Prog. 22, 1570–7.

L1101, L1102, L1061, L1081 Use of this vector for applications outside of the TNT® T7 Insect Cell Extract Protein Expression System may require a license from Shimadzu Corporation. For more information contact: t-direct@shimadzu-biotech.jp

L1101, L1102 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

L1061, L1081 For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

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