pCI Mammalian Expression Vector

The pCI Mammalian Expression Vector promotes constitutive expression of cloned DNA inserts in mammalian cells. The major difference between the pCI and pSI Mammalian Expression Vectors is the enhancer/promoter region controlling the expression of the inserted gene. The pCI Expression Vector contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region. This vector can be used for both transient and stable expression of genes. For stable expression, the pCI Vector must be co-transfected with an expression vector containing a selectable gene for mammalian cells.

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The pCI Mammalian Expression Vector promotes constitutive expression of cloned DNA inserts in mammalian cells. The major difference between the pCI and pSI Mammalian Expression Vectors is the enhancer/promoter region controlling the expression of the inserted gene. The pCI Expression Vector contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region. This vector can be used for both transient and stable expression of genes. For stable expression, the pCI Vector must be co-transfected with an expression vector containing a selectable gene for mammalian cells.

Features - Benefits

• Strong, Constitutive Expression: The pCI Vector's CMV enhancer/promoter region enables strong, constitutive expression in many cell types. A β-globin/IgG chimeric intron located downstream of the enhancer/promoter region can further increase expression.
• Increased Steady-State mRNA Levels: The late SV40 polyadenylation signal increases the steady-state level of RNA approximately fivefold more than the early SV40 polyadenylation signal.
• Convenient: Multiple cloning sites exist for easy insertion of cDNA.
• Versatile: Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication.

References

1. Brinster, R.L. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 836–40.
2. Choi, T. et al. (1991) Mol. Cell. Biol. 11, 3070–4.
3. Palmiter, R.D. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 478–82.
4. Carswell, S. and Alwine, J.C. (1989) Mol. Cell. Biol. 9, 4248–58.

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