QuantiFluor™-ST Fluorometer Method For DNA Quantitation Using Hoechst 33258
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Abstract
As with the common practice of visualizing DNA in a gel with ethidium bromide, quantitation of DNA can be easily achieved in a fluorometer with the dye, Hoechst 33258, a bisbenzimide DNA intercalator that excites in the near UV (350nm) and emits in the blue region (450nm). Sensitivity of the Hoechst 33258 assay is approximately 10ng/ml when it is used in conjunction with the QuantiFluor™-ST Fluorometer.