Detectable amounts of protein can be expressed from many commonly used T7-promoter-containing protein expression vectors using the TnT® T7 Quick Coupled Transcription/Translation System and your choice of non-radioactive labels. In this article, we summarize data generated using sixteen different protein expression vectors to express seven different target proteins, highlighting the ability of the TnT® T7 Quick Coupled Transcription/Translation System to screen protein expression vectors quickly and easily.
Promega Notes 101, 8–9.
Kate Qin Zhao
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