pF5K CMV-neo Flexi® Vector Product Information

Literature # 9PIC941

The pF5K CMV-neo Flexi® Vector is designed for use with the Flexi® System, Entry/Transfer (Cat.# C8640), and the Flexi® System, Transfer (Cat.# C8820). The pF5K CMV-neo Flexi® Vector contains the CMV immediate early enhancer/promoter region plus a chimeric intron for mammalian expression and a T7 promoter for in vitro expression of the protein-coding region. The vector also contains the barnase gene for positive selection of the insert, a kanamycin-resistance gene for selection of the plasmid, and unique SgfI and PmeI sites that allow easy insertion and transfer of the insert of interest. The pF5K CMV-neo Flexi® Vector also contains a neomycin-resistance gene under the control of the SV40 promoter for long-term selection of transfected mammalian cells. Transfection of a pF5K CMV-neo Flexi® Vector containing a cloned protein-coding region into mammalian cells provides resistance to the antibiotic G-418 (Cat.# V7981, V8091), allowing selection of transfectants.