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Rat Hepatocyte Culture Physiology Shows Enhanced Cytochrome P450 Activity on a...

Rat Hepatocyte Culture Physiology Shows Enhanced Cytochrome P450 Activity on a Synthetic Extracellular Matrix

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Abstract

We examined the impact of a new three-dimensional synthetic matrix on hepatocyte physiology. Hepatocytes were plated on Corning® labware with Ultra-Web™ and Ultra-Web™ polyamine surfaces and on Becton Dickinson (BD) BioCoat™ collagen I microplates. The samples were then challenged for 48 hours with two known modulators of cytochrome P450, and P450 activity was measured using the P450-Glo® Assay. The data show enhanced physiologic function of primary rat hepatocytes on microplates coated with the Ultra-Web™ synthetic matrix.

Cell Notes 20, 18–20.

Mark E. Rothenberg1, James J. Cali2, Mary Sobol2, Michael W. Briggs1 and Todd Upton1

1Technical Applications, Corning Life Sciences; 2Promega Corporation
Publication Date: 2008

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