Promega Corporation

Transfecting a Human Neuroblastoma Cell Line with Monster Green® Fluorescent Protein

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Abstract

We expressed an improved green fluorescent protein, Monster Green® Fluorescent Protein, in a human neuroblastoma cell line, SH-SY5Y. Optimized transfection was accomplished using TransFast™ Transfection Reagent. Expression of the Monster Green® Fluorescent Protein was bright and did not appear to interfere with the development of normal neuronal cell morphology.

Cell Notes 7, 14–16.

Natalie Betz and Abigail Farfan

Promega Corporation
Publication Date: 2003

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