Promega Corporation

Automated Triplex Assay to Assess Cell Viability Cytotoxicity and Apoptosis Scientific...

Automated Triplex Assay to Assess Cell Viability Cytotoxicity and Apoptosis Scientific Poster

Share

SEARCH POSTERS

Abstract

Sarah Shultz1, Andrew Niles1, Jason Greene2, Terry Riss1, Simon T. M. Allard1 and Paul Held2
1Promega Corporation, Madison, WI USA
2BioTek Instruments, Inc., Highland Park, P.O. Box 998, Winooski, Vermont, USA

Cell-based assays are useful tools to investigate in vitro responses to a variety of target compounds and cell-signaling molecules. Multiparametric measures are often desirable, particularly in secondary screening, to generate more biologically relevant data. The evaluation of multiple parameters can become quite labor intensive and time consuming if conducted in parallel, so the use of simple multiplex assays executed with automation help to improve efficiency and reduce operator involvement.

Here, we introduce an automated triplex cell-based assay application for simultaneously measuring three parameters: cell viability, cytotoxicity and apoptosis. The method combines two fluorescent and luminescent assay chemistries offered by Promega (Caspase-Glo® 3/7 and MultiTox-Fluor™ Assays) in the same assay well to extract information about viability, cytoxicity and caspase activation events. These parameters are particularly useful to define mechanisms associated with a cytotoxic profile. All dispensing steps are automated adn performed using the BioTek Precision™ XS Automated Sample Processor.

Document Icon Download the poster PDF (2112kb)

  • Part# PS081
  • Printed in USA.

Prefer a different language?

Your country is set to Brazil. Your language is set to Português. Please select the language that will best suit your needs:

This is correct, continue to site »

I need additional help

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.