Katharine Driftmier Miller Hoffmann1, Karen Reeces1, Cesear Corona2, Thomas Kirkland2, H. Tetsuo Uyeda2, Stephen J. Dwight, 2, Mark G. McDougal2 and Douglas R. Storts1
1Promega Corporation, Madison, WI
2Promega Biosciences, LLC, San Luis Obispo, CA
GoTaq® qPCR Master Mix introduces a new, proprietary dsDNA-binding dye that can be used at a higher concentration than SYBR® Green I because it is less inhibitory in an amplification reaction. The dye concentration in the master mix is optimized to produce significantly brighter fluorescence during qPCR than master mixes containing SYBR® Green I. Excitation and emission of the dye are similar to that of SYBR® Green I, so it is compatible with commonly available instrumentation platforms. GoTaq® qPCR Master Mix is a 2X master mix composed of an optimized buffer formulation complete with dNTPs and MgCl2 and features GoTaq® Hot Start. The master mix comes premixed with a low level of CXR reference dye, which is identical to ROX™ reference dye. The master mix is compatible with existing experimental designs and requires addition of only DNA template, target-specific primers and water.