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LC-MRM Quantification of Src-Family Kinases using Protein Standards Expressed in...

LC-MRM Quantification of Src-Family Kinases using Protein Standards Expressed in Cell-Free Systems Poster

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Abstract

Elizabeth Remily-Wood1, Michael Rosenblatt2, Robin Hurst2, Fumi Kinose1, Eric Haura1, Mark Meads1 ,William Dalton1, Kenneth Shain,1 John Koomen1
1H. Lee Moffitt Cancer Center, Tampa, FL; 2Promega, Madison, WI

The SRC family of tyrosine kinases includes members with high protein sequence homology (Src, Fyn, Yes, Lck, Hck, Lyn, Blk, Fgr, Frk, and SRMS). Signaling mediated by these proteins control cell proliferation and migration; thus, they play major roles in cancer development and progression. Detailed examination of SRC family kinases (SFKs) can elucidate cancer biology, and peptide-based quantitative assays for the expression of each SFK can have utility in deciphering which of the family members is expressed in tumor cells.

LC-MRM assays can quantify the expression of each SFK using unique peptides from each sequence. Applied in parallel, all SFK proteins can be detected and quantified in a single experiment, providing additional utility over immunoblotting. Expressed proteins aid assay development and serve as stable isotope-labeled standards (SIS).

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  • Part# PS157
  • Printed in USA.

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