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J. Immunol. 162, 2422-2426. A conformation-dependent epitope in Addison's disease and other endocrinological autoimmune diseases maps to a carboxyl-terminal functional domain of human steroid 21-hydroxylase. 1999

Nikoshkov, A., Falorni, A., Lajic, S., Laureti, S., Wedell, A., Lernmark, Å, Luthman, H.

Notes: The pGEM®-3Z Vector was used in subcloning. (0614)

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pGEM®-3Z Vector

Proc. Natl. Acad. Sci. USA 96, 9051-9056. Direct evidence that the rifamycin polyketide synthase assembles polyketide chains processively. 1999

Yu, T.-W., Shen, Y., Doi-Katayama, Y., Tang, L., Park, C., Moore, B.S., Hutchinson, C.R., Floss, H.G.

Notes: The pGEM®-3Zf(+) Vector was used for routine subcloning. (0077)

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pGEM®-3Z Vector

J. Gen. Virol. 80, 1929-1941. Sequence analysis of a porcine enterovirus serotype 1 isolate: Relationships with other picornaviruses. 1999

Doherty, M., Todd, D., McFerran, N., Hoey, E.M.

Notes: RNA was isolated from purified porcine enterovirus serotype 1 and oligo-d(T) primed, double stranded cDNA synthesis was accomplished using the Universal Riboclone® cDNA Synthesis System. The dsDNA was subcloned into the pGEM®-3Z Vector using of EcoRI Adaptors. (1239)

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J. Biol. Chem. 274, 24567-24574. Skp, a molecular chaperone of gram-negative bacteria, is required for the formation of soluble periplasmic intermediates of outer membrane proteins. 1999

Schäfer, U., Beck, K., Müller, M.

Notes: The pGEM®-3Z Vector was used for subcloning. (0448)

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J. Immunol. 163, 2008-2016. Specific inhibition of macrophage TNFα expression by in vivo ribozyme treatment. 1999

Kisich, K.O., Malone, R.W., Feldstein, P.A., Erickson, K.L.

Notes: The pGEM®-3Z Vector was used for subcloning the cDNA for the ribozyme and was the template for in vitro transcription. (0904)

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pGEM®-3Z Vector

J. Immunol. 163, 2008–2016. Specific inhibition of macrophage TNF-α expression by in vivo ribozyme treatment. 1999

Kisich, K.O., Malone, R.W., Feldstein, P.A., and Erickson, K.L.

Notes: Ribozymes designed to cleave TNF-α cDNA were cloned into the pGEM®-3Z Vector before being transcribed in vitro. The transcribed ribozymes were then mixed with Transfectam® Reagent and injected into mice intraperitoneally. Tissues and cells isolated from the mice were assayed at later time points for fluor or radiolabled ribozymes. In vitro kinetic assays were also performed with ribozymes and RNA substrates labeled with T4 Polynucleotide Kinase.  (2834)

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Blood 89, 1394-1404. Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1. 1997

Pulford, K., Lamant, L., Morris, S.W., Butler, L.H., Wood, K.M., Stroud, D., Delsol, G., Mason, D.Y.

Notes: The ProFection® Mammalian Transfection System-CaPO4 was used to transiently transfect 293T cells. (0521)

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