N195 pFN35K SmBiT TK-neo Flexi Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 N195 pFN35K SmBiT TK-neo Flexi Vector Vector sequence reference points: Base pairs 4421 HSV-TK promoter 1-753 SmBiT 802-837 SgfI Site 883-890 Barnase coding region 914-1249 PmeI site 1251-1258 Sv40 late polyadenylation signal 1410-1631 SV40 enhancer and early promoter 1730-2148 SV40 Enhancer 1803-2039 (Reverse) Sv40 Min Ori 2046-2111 EM7 bacterial promoter 2156-2222 Neo-Kan Resistance 2236-3030 Synthetic PolyAdenylation Signal Sequence 3094-3142 ColE1-derived plasmid replication origin 3378-3414 ---- 1 AAATGAGTCT TCGGACCTCG CGGGGGCCGC TTAAGCGGTG GTTAGGGTTT 51 GTCTGACGCG GGGGGAGGGG GAAGGAACGA AACACTCTCA TTCGGAGGCG 101 GCTCGGGGTT TGGTCTTGGT GGCCACGGGC ACGCAGAAGA GCGCCGCGAT 151 CCTCTTAAGC ACCCCCCCGC CCTCCGTGGA GGCGGGGGTT TGGTCGGCGG 201 GTGGTAACTG GCGGGCCGCT GACTCGGGCG GGTCGCGCGC CCCAGAGTGT 251 GACCTTTTCG GTCTGCTCGC AGACCCCCGG GCGGCGCCGC CGCGGCGGCG 301 ACGGGCTCGC TGGGTCCTAG GCTCCATGGG GACCGTATAC GTGGACAGGC 351 TCTGGAGCAT CCGCACGACT GCGGTGATAT TACCGGAGAC CTTCTGCGGG 401 ACGAGCCGGG TCACGCGGCT GACGCGGAGC GTCCGTTGGG CGACAAACAC 451 CAGGACGGGG CACAGGTACA CTATCTTGTC ACCCGGAGGC GCGAGGGACT 501 GCAGGAGCTT CAGGGAGTGG CGCAGCTGCT TCATCCCCGT GGCCCGTTGC 551 TCGCGTTTGC TGGCGGTGTC CCCGGAAGAA ATATATTTGC ATGTCTTTAG 601 TTCTATGATG ACACAAACCC CGCCCAGCGT CTTGTCATTG GCGAAGTCGA 651 ACACGCAGAT GCAGTCGGGG CGGCGCGGTC CCAGGTCCAC TTCGCATATT 701 AAGGTGACGC GTGTGGCCTC GAACACCGAG CGACCCTGCA GCGACCCGCT 751 TAAAAGCTTG GCAATCCGGT ACTGTTGGTA AAGCCACCGC TAGCGCTCAC 801 CATGGTGACC GGCTACCGGC TGTTCGAGGA GATTCTCGGG AGCTCCGGTG 851 GTGGCGGGAG CGGAGGTGGA GGCTCGAGCG GTGCGATCGC CATGGAATAA 901 GTAAGGAATC CACATGGCAC AGGTTATCAA CACGTTTGAC GGGGTTGCGG 951 ATTATCTTCA GACATATCAT AAGCTACCTG ATAATTACAT TACAAAATCA 1001 GAAGCACAAG CCCTCGGCTG GGTGGCATCA AAAGGGAACC TTGCAGACGT 1051 CGCTCCGGGG AAAAGCATCG GCGGAGACAT CTTCTCAAAC AGGGAAGGCA 1101 AACTCCCGGG CAAAAGCGGA CGAACATGGC GTGAAGCGGA TATTAACTAT 1151 ACATCAGGCT TCAGAAATTC AGACCGGATT CTTTACTCAA GCGACTGGCT 1201 GATTTACAAA ACAACGGACC ATTATCAGAC CTTTACAAAA ATCAGATAAT 1251 GTTTAAACGA ATTCGGGCTC GGTACCCGGG GATCCTCTAG AGTCGACCTG 1301 CAGGCATGCA AGCTGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG 1351 TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCGGC 1401 CGCTTCGAGC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA 1451 ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT 1501 TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA 1551 ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGATGTG GGAGGTTTTT 1601 TTAAGCAAGT AAAACCTCTA CAAATGTGGT AAAATCGAAT TTTAACAAAA 1651 TATTAACGCT TACAATTTCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT 1701 GCGGTATTTC ACACCGCATA CGCGGATCTG CGCAGCACCA TGGCCTGAAA 1751 TAACCTCTGA AAGAGGAACT TGGTTAGGTA CCTTCTGAGG CGGAAAGAAC 1801 CAGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC 1851 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT 1901 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 1951 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC 2001 CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT 2051 TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 2101 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTAA 2151 TTAACTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA 2201 CGACAAGGTG AGGAACTAAA CCCAGGAGGC AGATCATGAT TGAACAAGAT 2251 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA 2301 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 2351 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 2401 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC 2451 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 2501 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 2551 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG 2601 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 2651 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 2701 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC 2751 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 2801 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 2851 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC 2901 GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 2951 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 3001 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA 3051 ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGATGGC CGCAATAAAA 3101 TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGAATCGATA 3151 GCGATAAGGA TCCTCTTTGC GCTTGCGTTT TCCCTTGTCC AGATAGCCCA 3201 GTAGCTGACA TTCATCCGGG GTCAGCACCG TTTCTGCGGA CTGGCTTTCT 3251 ACCCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA 3301 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 3351 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 3401 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 3451 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 3501 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 3551 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 3601 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 3651 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 3701 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 3751 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 3801 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA 3851 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 3901 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 3951 CAGAAAAAAA GGATTTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 4001 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 4051 TCAAAAAGGA TCTTCACCTA GATCCTTTTA TAGTCCGGAA ATACAGGAAC 4101 GCACGCTGGA TGGCCCTTCG CTGGGATGGT GAAACCATGA AAAATGGCAG 4151 CTTCAGTGGA TTAAGTGGGG GTAATGTGGC CTGTACCCTC TGGTTGCATA 4201 GGTATTCATA CGGTTAAAAT TTATCAGGCG CGATTGCGGC AGTTTTTCGG 4251 GTGGTTTGTT GCCATTTTTA CCTGTCTGCT GCCGTGATCG CGCTGAACGC 4301 GTTTTAGCGG TGCGTACAAT TAAGGGATTA TGGTAAATCC ACTTACTGTC 4351 TGCCCTCGTA GCCATCGAGA TAAACCGCAG TACTCCGGCC ACGATGCGTC 4401 CGGCGTAGAG GATCGAGATC T Sequence file prepared 11/20/2015 by Feature database and feature finder version 1.0.4.2.