N193 pFC36K SmBiT TK-neo Flexi Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 N193 pFC36K SmBiT TK-neo Flexi Vector Vector sequence reference points: Base pairs 4412 HSV-TK promoter 1-753 SgfI Site 793-800 Barnase coding region 824-1159 EcoICR I 1179-1184 SmBiT 1232-1264 Sv40 late polyadenylation signal 1401-1622 SV40 enhancer and early promoter 1721-2139 SV40 Enhancer 1794-2030 (Reverse) Sv40 Min Ori 2037-2102 EM7 bacterial promoter 2147-2213 Neo-Kan Resistance 2227-3021 Synthetic PolyAdenylation Signal Sequence 3085-3133 ColE1-derived plasmid replication origin 3369-3405 ---- 1 AAATGAGTCT TCGGACCTCG CGGGGGCCGC TTAAGCGGTG GTTAGGGTTT 51 GTCTGACGCG GGGGGAGGGG GAAGGAACGA AACACTCTCA TTCGGAGGCG 101 GCTCGGGGTT TGGTCTTGGT GGCCACGGGC ACGCAGAAGA GCGCCGCGAT 151 CCTCTTAAGC ACCCCCCCGC CCTCCGTGGA GGCGGGGGTT TGGTCGGCGG 201 GTGGTAACTG GCGGGCCGCT GACTCGGGCG GGTCGCGCGC CCCAGAGTGT 251 GACCTTTTCG GTCTGCTCGC AGACCCCCGG GCGGCGCCGC CGCGGCGGCG 301 ACGGGCTCGC TGGGTCCTAG GCTCCATGGG GACCGTATAC GTGGACAGGC 351 TCTGGAGCAT CCGCACGACT GCGGTGATAT TACCGGAGAC CTTCTGCGGG 401 ACGAGCCGGG TCACGCGGCT GACGCGGAGC GTCCGTTGGG CGACAAACAC 451 CAGGACGGGG CACAGGTACA CTATCTTGTC ACCCGGAGGC GCGAGGGACT 501 GCAGGAGCTT CAGGGAGTGG CGCAGCTGCT TCATCCCCGT GGCCCGTTGC 551 TCGCGTTTGC TGGCGGTGTC CCCGGAAGAA ATATATTTGC ATGTCTTTAG 601 TTCTATGATG ACACAAACCC CGCCCAGCGT CTTGTCATTG GCGAAGTCGA 651 ACACGCAGAT GCAGTCGGGG CGGCGCGGTC CCAGGTCCAC TTCGCATATT 701 AAGGTGACGC GTGTGGCCTC GAACACCGAG CGACCCTGCA GCGACCCGCT 751 TAAAAGCTTG GCAATCCGGT ACTGTTGGTA AAGCCACCGC TAGCGATCGC 801 CATGGAATAA GTAAGGAATC CACATGGCAC AGGTTATCAA CACGTTTGAC 851 GGGGTTGCGG ATTATCTTCA GACATATCAT AAGCTACCTG ATAATTACAT 901 TACAAAATCA GAAGCACAAG CCCTCGGCTG GGTGGCATCA AAAGGGAACC 951 TTGCAGACGT CGCTCCGGGG AAAAGCATCG GCGGAGACAT CTTCTCAAAC 1001 AGGGAAGGCA AACTCCCGGG CAAAAGCGGA CGAACATGGC GTGAAGCGGA 1051 TATTAACTAT ACATCAGGCT TCAGAAATTC AGACCGGATT CTTTACTCAA 1101 GCGACTGGCT GATTTACAAA ACAACGGACC ATTATCAGAC CTTTACAAAA 1151 ATCAGATAAT GTTTAATGAC CCCGTGTCGA GCTCAAGGCA GTTCAGGTGG 1201 TGGCGGGAGC GGAGGTGGAG GCTCGAGCGG TGTGACCGGC TACCGGCTGT 1251 TCGAGGAGAT TCTGTAAGGC CGCGACTCTA GAGTCGACCT GCAGGCATGC 1301 AAGCTGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT 1351 GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCGG CCGCTTCGAG 1401 CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG 1451 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT 1501 TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC 1551 ATTTTATGTT TCAGGTTCAG GGGGAGATGT GGGAGGTTTT TTTAAGCAAG 1601 TAAAACCTCT ACAAATGTGG TAAAATCGAA TTTTAACAAA ATATTAACGC 1651 TTACAATTTC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT 1701 CACACCGCAT ACGCGGATCT GCGCAGCACC ATGGCCTGAA ATAACCTCTG 1751 AAAGAGGAAC TTGGTTAGGT ACCTTCTGAG GCGGAAAGAA CCAGCTGTGG 1801 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG 1851 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT 1901 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 1951 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC 2001 GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT 2051 ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 2101 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTA ATTAACTGTT 2151 GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT 2201 GAGGAACTAA ACCCAGGAGG CAGATCATGA TTGAACAAGA TGGATTGCAC 2251 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC 2301 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC 2351 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 2401 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT 2451 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC 2501 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 2551 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC 2601 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG 2651 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 2701 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA 2751 GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT 2801 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 2851 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC 2901 CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG 2951 TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 3001 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC 3051 CAAGCGACGC CCAACCTGCC ATCACGATGG CCGCAATAAA ATATCTTTAT 3101 TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT AGCGATAAGG 3151 ATCCTCTTTG CGCTTGCGTT TTCCCTTGTC CAGATAGCCC AGTAGCTGAC 3201 ATTCATCCGG GGTCAGCACC GTTTCTGCGG ACTGGCTTTC TACCCGGTAT 3251 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 3301 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 3351 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 3401 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 3451 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 3501 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 3551 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 3601 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 3651 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 3701 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 3751 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 3801 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 3851 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 3901 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 3951 AGGATTTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 4001 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 4051 ATCTTCACCT AGATCCTTTT ATAGTCCGGA AATACAGGAA CGCACGCTGG 4101 ATGGCCCTTC GCTGGGATGG TGAAACCATG AAAAATGGCA GCTTCAGTGG 4151 ATTAAGTGGG GGTAATGTGG CCTGTACCCT CTGGTTGCAT AGGTATTCAT 4201 ACGGTTAAAA TTTATCAGGC GCGATTGCGG CAGTTTTTCG GGTGGTTTGT 4251 TGCCATTTTT ACCTGTCTGC TGCCGTGATC GCGCTGAACG CGTTTTAGCG 4301 GTGCGTACAA TTAAGGGATT ATGGTAAATC CACTTACTGT CTGCCCTCGT 4351 AGCCATCGAG ATAAACCGCA GTACTCCGGC CACGATGCGT CCGGCGTAGA 4401 GGATCGAGAT CT Sequence file prepared 11/20/2015 by Feature database and feature finder version 1.0.4.2.