N204 SmBiT-PRKACA Control Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 N204 SmBiT-PRKACA Control Vector Vector sequence reference points: Base pairs 5115 HSV-TK promoter 1-753 SmBiT-PRKACA 802-1944 Sv40 late polyadenylation signal 2104-2325 SV40 enhancer and early promoter 2424-2842 SV40 Enhancer 2497-2733 (Reverse) Sv40 Min Ori 2740-2805 EM7 bacterial promoter 2850-2916 Neo-Kan Resistance 2930-3724 Synthetic PolyAdenylation Signal Sequence 3788-3836 ColE1-derived plasmid replication origin 4072-4108 ---- 1 AAATGAGTCT TCGGACCTCG CGGGGGCCGC TTAAGCGGTG GTTAGGGTTT 51 GTCTGACGCG GGGGGAGGGG GAAGGAACGA AACACTCTCA TTCGGAGGCG 101 GCTCGGGGTT TGGTCTTGGT GGCCACGGGC ACGCAGAAGA GCGCCGCGAT 151 CCTCTTAAGC ACCCCCCCGC CCTCCGTGGA GGCGGGGGTT TGGTCGGCGG 201 GTGGTAACTG GCGGGCCGCT GACTCGGGCG GGTCGCGCGC CCCAGAGTGT 251 GACCTTTTCG GTCTGCTCGC AGACCCCCGG GCGGCGCCGC CGCGGCGGCG 301 ACGGGCTCGC TGGGTCCTAG GCTCCATGGG GACCGTATAC GTGGACAGGC 351 TCTGGAGCAT CCGCACGACT GCGGTGATAT TACCGGAGAC CTTCTGCGGG 401 ACGAGCCGGG TCACGCGGCT GACGCGGAGC GTCCGTTGGG CGACAAACAC 451 CAGGACGGGG CACAGGTACA CTATCTTGTC ACCCGGAGGC GCGAGGGACT 501 GCAGGAGCTT CAGGGAGTGG CGCAGCTGCT TCATCCCCGT GGCCCGTTGC 551 TCGCGTTTGC TGGCGGTGTC CCCGGAAGAA ATATATTTGC ATGTCTTTAG 601 TTCTATGATG ACACAAACCC CGCCCAGCGT CTTGTCATTG GCGAAGTCGA 651 ACACGCAGAT GCAGTCGGGG CGGCGCGGTC CCAGGTCCAC TTCGCATATT 701 AAGGTGACGC GTGTGGCCTC GAACACCGAG CGACCCTGCA GCGACCCGCT 751 TAAAAGCTTG GCAATCCGGT ACTGTTGGTA AAGCCACCGC TAGCGCTCAC 801 CATGGTGACC GGCTACCGGC TGTTCGAGGA GATTCTCGGG AGCTCCGGTG 851 GTGGCGGGAG CGGAGGTGGA GGCTCGAGCG GTGCGATCGC CATGGGCAAC 901 GCCGCCGCCG CCAAGAAGGG CAGCGAGCAG GAGAGCGTGA AAGAATTCTT 951 AGCCAAAGCC AAAGAAGATT TTCTTAAAAA ATGGGAAAGT CCCGCTCAGA 1001 ACACAGCCCA CTTGGATCAG TTTGAACGAA TCAAGACCCT CGGCACGGGC 1051 TCCTTCGGGC GGGTGATGCT GGTGAAACAC AAGGAGACCG GGAACCACTA 1101 TGCCATGAAG ATCCTCGACA AACAGAAGGT GGTGAAACTG AAACAGATCG 1151 AACACACCCT GAATGAAAAG CGCATCCTGC AAGCTGTCAA CTTTCCGTTC 1201 CTCGTCAAAC TCGAGTTCTC CTTCAAGGAC AACTCAAACT TATACATGGT 1251 CATGGAGTAC GTGCCCGGCG GGGAGATGTT CTCACACCTA CGGCGGATCG 1301 GAAGGTTCAG TGAGCCCCAT GCCCGTTTCT ACGCGGCCCA GATCGTCCTG 1351 ACCTTTGAGT ATCTGCACTC GCTGGATCTC ATCTACAGGG ACCTGAAGCC 1401 GGAGAATCTG CTCATTGACC AGCAGGGCTA CATTCAGGTG ACAGACTTCG 1451 GTTTCGCCAA GCGCGTGAAG GGCCGCACTT GGACCTTGTG CGGCACCCCT 1501 GAGTACCTGG CCCCTGAGAT TATCCTGAGC AAAGGCTACA ACAAGGCCGT 1551 GGACTGGTGG GCCCTGGGGG TTCTTATCTA TGAAATGGCC GCTGGCTACC 1601 CGCCCTTCTT CGCAGACCAG CCCATCCAGA TCTATGAGAA GATCGTCTCT 1651 GGGAAGGTGC GCTTCCCTTC CCACTTCAGC TCTGACTTGA AGGACCTGCT 1701 GCGGAACCTC CTGCAGGTAG ATCTCACCAA GCGCTTTGGG AACCTCAAGA 1751 ATGGGGTCAA CGATATCAAG AACCACAAGT GGTTTGCCAC AACTGACTGG 1801 ATTGCCATCT ACCAGAGGAA GGTGGAAGCT CCCTTCATAC CAAAGTTTAA 1851 AGGCCCTGGG GATACGAGTA ACTTTGACGA CTATGAGGAA GAAGAAATCC 1901 GGGTCTCCAT CAATGAGAAG TGTGGCAAGG AGTTTTCTGA GTTTGTTTAA 1951 ACGAATTCGG GCTCGGTACC CGGGGATCCT CTAGAGTCGA CCTGCAGGCA 2001 TGCAAGCTGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT 2051 GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CGGCCGCTTC 2101 GAGCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA 2151 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT 2201 ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA 2251 TTCATTTTAT GTTTCAGGTT CAGGGGGAGA TGTGGGAGGT TTTTTTAAGC 2301 AAGTAAAACC TCTACAAATG TGGTAAAATC GAATTTTAAC AAAATATTAA 2351 CGCTTACAAT TTCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA 2401 TTTCACACCG CATACGCGGA TCTGCGCAGC ACCATGGCCT GAAATAACCT 2451 CTGAAAGAGG AACTTGGTTA GGTACCTTCT GAGGCGGAAA GAACCAGCTG 2501 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 2551 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA 2601 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 2651 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC 2701 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA 2751 TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 2801 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TTAATTAACT 2851 GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA 2901 GGTGAGGAAC TAAACCCAGG AGGCAGATCA TGATTGAACA AGATGGATTG 2951 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG 3001 GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG 3051 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 3101 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG 3151 CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT 3201 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 3251 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA 3301 TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA 3351 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 3401 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT 3451 CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG 3501 CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 3551 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC 3601 TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC 3651 TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 3701 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC 3751 GACCAAGCGA CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT 3801 TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAATC GATAGCGATA 3851 AGGATCCTCT TTGCGCTTGC GTTTTCCCTT GTCCAGATAG CCCAGTAGCT 3901 GACATTCATC CGGGGTCAGC ACCGTTTCTG CGGACTGGCT TTCTACCCGG 3951 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 4001 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 4051 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 4101 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 4151 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 4201 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 4251 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 4301 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 4351 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 4401 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 4451 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 4501 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 4551 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 4601 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 4651 AAAAGGATTT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 4701 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 4751 AGGATCTTCA CCTAGATCCT TTTATAGTCC GGAAATACAG GAACGCACGC 4801 TGGATGGCCC TTCGCTGGGA TGGTGAAACC ATGAAAAATG GCAGCTTCAG 4851 TGGATTAAGT GGGGGTAATG TGGCCTGTAC CCTCTGGTTG CATAGGTATT 4901 CATACGGTTA AAATTTATCA GGCGCGATTG CGGCAGTTTT TCGGGTGGTT 4951 TGTTGCCATT TTTACCTGTC TGCTGCCGTG ATCGCGCTGA ACGCGTTTTA 5001 GCGGTGCGTA CAATTAAGGG ATTATGGTAA ATCCACTTAC TGTCTGCCCT 5051 CGTAGCCATC GAGATAAACC GCAGTACTCC GGCCACGATG CGTCCGGCGT 5101 AGAGGATCGA GATCT Sequence file prepared 11/20/2015 by Feature database and feature finder version 1.0.4.2.