Kinase Selectivity Profiling Systems—General Panel
Selectivity Screening of Library Compounds for Effect on Panels of Kinases
- 24 kinase and substrate pairs organized in three 8-tube strips
- Simple reaction assembly and automation-friendly protocol
- Streamlines profiling of hits and leads for potency against families of related kinases
Catalog Number:
Choose a kinase profiling system
Additional options
Profile Lead Compounds in Hours, Not Days
The Kinase Selectivity Profiling Systems are ideal for profiling lead-compound kinase inhibitors against a broad panel of kinases to better understand inhibitor activity and obviate any off-target effects. Each system includes kinase and substrate pairs organized in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.
The General Panel Kinase Selectivity Profiling System contains 24 kinases arranged in three 8-well strips, the corresponding substrates and cofactors, and kinase reaction buffer.
Optimized for Use With ADP-Glo™ Kinase Assay
The Kinase Selectivity Profiling Systems rely on the luminescent ADP-Glo™ Kinase Assay technology to characterize kinase selectivity. The luminescence generated by the ADP-Glo™ Kinase Assay is proportional to ADP concentration and correlates with kinase activity.
Each Kinase Selectivity Profiling System is available with or without ADP-Glo™ Assay reagents.
Simple, Automation-Friendly Assay, No Time-Consuming Optimization
Simply dilute the stock solutions provided, and you’re ready to go. Two quick, one-step dilutions provide working stocks of kinases and substrate/cofactor solutions.
Concentrated kinases and substrates/cofactor stocks are first diluted and then combined with test compound in a 384-well plate. After a 1-hour incubation, the ADP-Glo™ Assay is performed to measure kinase activity.
Assays can be performed manually, or automated on a liquid-handler.
Single-Dose Profiling
Protocols
Specifications
Catalog Number:
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size |
|---|---|---|
Kinase Selectivity Profiling System: General Panel |
V6928 | 1 × 24 × 50 reactions |
ADP-Glo™ Kinase Assay |
V6930 | 3 × 400 assays |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
Resources
No related resources available
