SmaI
- Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
- Genome qualified to ensure optimal performance in genomic analysis applications
Catalog Number:
Choose a concentration
Size
Catalog Number: R6121
Catalog Number: R6125
Cut Site:
CCC▼GGG
GGG▲CCC
Note: XmaI is a neoschizomer of SmaI.
Incubation Conditions: Buffer J. 25°C. (SmaI exhibits 25–50% activity at 37°C.)
Source: Serratia marcescens.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | J | MULTI-CORE™ |
|---|---|---|---|---|---|
| <10% | <10% | <10% | <10% | 100% | 100% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 3 | 12 | 0 | 1 | 1 | 0 |
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer J 10X Buffer |
R009A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
SmaI |
R612A | 1 × 1,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer J 10X Buffer |
R009A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396E | 1 × 750μl | 10mg/ml | View Product |
|
SmaI |
R612F | 1 × 5,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Other Resources
Related Products
Frequently Used With
LigaFast™ Rapid DNA Ligation System
Efficiently ligates DNA inserts into plasmid vectors in just 5–15 minutes at room temperature.
M8221, M8225
Blue/Orange Loading Dye, 6X
Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.
G1881
Agarose, LE, Analytical Grade
Reagent for electrophoretic separation of nucleic acids.
V3121, V3125
Ethidium Bromide Solution, Molecular Grade
Fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients.
H5041
