Lys-C, Mass Spec Grade
Protease for Challenging Protein Digestions
- Active under strong denaturing conditions
- C-terminal (Lys-C) lysine cleavage
- Generates longer peptides than with tryptic protein digestion
Catalog Number:
Size
Catalog Number: VA1170
Active Under Strong Denaturing Conditions
Endoproteinase Lys-C, Mass Spec Grade, is a highly-purified serine protease that hydrolyzes specifically at the carboxyl side of lysines. Lys-C retains proteolytic activity under strong protein denaturing conditions such as 8M urea, which can be used to improve digestion of proteolytically resistant proteins. Lys-C, Mass Spec Grade, has optimal activity in the range of pH 7.0—9.0. This protease can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching.
Digestion Under Strong Denaturing Conditions
Native Lys-C Interchangeable with Recombinant Lys-C
Comparison of lysine-specific cleavages. Mass Spec-Compatible Yeast Protein Extract, Intact (Cat.# V7341) was digested with either rLys-C (P. aeruginosa; Cat.# V1671) or native Lys-C (Lysobacter sp.; Cat.# VA1170) at a ratio of 1:100.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
Lys-C Mass Spec Grade |
VA117A | 1 × 20μg |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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