pGL4.82[hRluc/Puro] Vector Protocol
Instructions for Use of Product(s)
E7501
Literature # 9PIE750
The pGL4.82[hRluc/Puro] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for puromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. It has also been engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.82[hRluc/Puro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice.
Printed in USA. Revised 10/16.