Lumit® IFN-γ (Human) Immunoassay
No-Wash Immunoassay for IFN-γ Detection
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a plate-reading luminometer
- Scalable to 96- or 384- well plates, protocols included
- Pack sizes show assay number for 96-well plates
Catalog Number:
Choose a product
Size
Catalog Number: W6040
Catalog Number: W6042
Catalog Number: W6041
Catalog Number: W134A-C
Quantifique a Liberação de Citocinas sem as Tediosas Etapas de Lavagem
O imunoensaio Lumit™ IFN-γ mede quantitativamente o IFN-γ liberado em amostras de cultura de células usando um protocolo simples e sem lavagem. Basta adicionar anticorpos marcados à amostra, adicionar o reagente de detecção e ler o sinal luminescente usando um luminômetro de leitura de placa padrão. Todo o protocolo é concluído em menos de 70 minutos! O ensaio pode ser usado diretamente nas células em cultura ou no meio de cultura transferido para uma placa de ensaio separada.
Como Funciona o Imunoensaio Lumit™ IFN-γ
Os anticorpos primários para IFN-γ foram selecionados por sua detecção específica e sensível e marcados com as subunidades LgBiT e SmBiT da NanoBiT® Luciferase. Na presença de IFN-γ, as subunidades são reunidas para formar uma enzima luciferase ativa. A adição de substrato otimizado gera um sinal luminescente brilhante proporcional aos níveis de IFN-γ.
Protocolo Simples de Uma Placa, Sem Etapas de Lavagem
Grande Faixa Dinâmica da Detecção de IFN-γ do Lumit™
A faixa linear de pg/ml a ng/ml da detecção do Lumit™ abrange as concentrações esperadas para a maioria dos modelos de células.
|
Assay |
IFN-γ Human Immunoassay |
|
|
Limit of Detection (LOD) |
1.7pg/ml (3 SD above background) |
|
|
Dynamic Range |
7.3pg/ml – 10ng/ml |
|
|
Minimal Detectable Dose (MDD) |
1.1pg/ml (2 SD above background) |
|
|
Assay Time |
70 minutes |
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Sample Type |
Cell culture supernatants |
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SD=Standard Deviation
Detectar IFN-γ como um Marcador de Ativação de Células T
Purified CD8+ T cells (effector cells) were combined with Raji B cells (target cells) and a serial dilution of Blincyto® (CD3 and CD19 bispecific T cell engager). The Lumit® IFN-γ (Human) Immunoassay reagents were added directly to treated cell wells to detect IFN-γ release from the T cells.
Detecção de Várias Citocinas por Análise de Amostra Dividida
Human PBMCs were plated in a 96-well plate in 100µl and treated for 24 hours with cell stimulation cocktail (CSC), lipopolysaccharide (LPS) or R848. Four 20μl aliquots were transferred to a 384-well plate for detection of IL-2, IL-6, IFN-γ and TNF-α with the corresponding Lumit® Cytokine Immunoassay. This simple approach for profiling cytokine release can be applied to multiple cell models.
Explore Outros Ensaios de Detecção de Citocinas
Oferecemos um crescente portfólio de ensaios para detecção de citocinas em amostras de cultura de células.
Não’ encontrar seu alvo na lista?
Por favor, informe-se sobre o acesso antecipado a novos ensaios em desenvolvimento.
Para uma empresa de biotecnologia que entende que o tempo tem um valor significativo, os imunoensaios Lumit™ são uma forma essencial de coletar dados confiáveis rapidamente para que possamos chegar a conclusões e tomar decisões mais rapidamente.
Jonathan Chow, Principal Scientist, Corner Therapeutics
Featured Application Note
CAR T cell-mediated cytotoxicity and cytokine release in response to antigen expression on target cells
Explore a multiplexing approach that unlocks the full potential of CAR T cell therapy with our Lumit® IFN-γ and TNF-α (Human) Immunoassays.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Código | Tamanho |
|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml |
Lumit® Human IFN-ɣ Standard |
W134A-C | 1 × 25μl |
Anti-hIFN-γ mAb-SmBiT, 500X |
W135A | 1 × 30μl |
Anti-hIFN-γ mAb-LgBiT, 500X |
W136A | 1 × 30μl |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Código | Tamanho |
|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml |
Lumit® Human IFN-ɣ Standard |
W134A-C | 1 × 25μl |
Anti-hIFN-γ mAb-SmBiT, 500X |
W135A | 5 × 30μl |
Anti-hIFN-γ mAb-LgBiT, 500X |
W136A | 5 × 30μl |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Código | Tamanho |
|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml |
Lumit® Human IFN-ɣ Standard |
W134A-C | 1 × 25μl |
Anti-hIFN-γ mAb-SmBiT, 500X |
W135B | 1 × 300μl |
Anti-hIFN-γ mAb-LgBiT, 500X |
W136B | 1 × 300μl |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Código | Tamanho |
|---|---|---|
Lumit® Human IFN-ɣ Standard |
W134A-C | 1 × 25μl |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
Resources
Outros recursos
- Rapid and Sensitive Determination of Cytokine Release from Cells Without Sample Transfer
- Blog Article: Understanding Inflammation: A Faster, Easier Way to Detect Cytokines in Cells
- Blog Article: The Cytokine Storm: Why Some COVID-19 Cases are More Severe
- White Paper: Lumit® Immunoassays: A Rapid and Sensitive Method for Analyte Detection
- Application Note: Automated Lumit™ IFN-γ (Human) Immunoassay on the Hamilton Microlab STARlet
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