Lumit® IL-4 (Human) Immunoassay
No-Wash Immunoassay for IL-4 Detection
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a plate-reading luminometer
- Scalable to 96- or 384- well plates, protocols included
- Pack sizes show assay number for 96-well plates
Catalog Number:
Choose a product
Size
Catalog Number: W6060
Catalog Number: W6062
Catalog Number: W6061
Catalog Number: W125A-C
Quantify Cytokine Release Without Tedious Wash Steps
The Lumit® IL-4 Immunoassay quantitatively measures released IL-4 in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.
Note: The total number of assays per kit will vary based on final well volume.
For standard well volumes:
- 100 assay kits: applicable for 100 assays in 96-well plates or 400 assays in 384-well plates.
- 500 assay kits: applicable for 500 assays in 96-well plates or 2,000 assays in 384-well plates.
- 1,000 assay kits: applicable for 1,000 assays in 96-well plates or 4,000 assays in 384-well plates.
Como Funciona o Imunoensaio Lumit™ IL-4
Os anticorpos primários para IL-4 foram selecionados por sua detecção específica e sensível e marcados com as subunidades LgBiT e SmBiT da NanoBiT® Luciferase. Na presença de IL-4, as subunidades são reunidas para formar uma enzima luciferase ativa. A adição de substrato otimizado gera um sinal luminescente brilhante proporcional aos níveis de IL-4.
Protocolo Simples de Uma Placa, Sem Etapas de Lavagem
Ampla Faixa Dinâmica da Detecção de IL-4 Lumit™
A faixa linear de pg/ml a ng/ml da detecção do Lumit™ abrange as concentrações esperadas para a maioria dos modelos de células.
|
Assay |
IL-4 Human Immunoassay |
|
|
Limit of Detection (LOD) |
6.7pg/ml (3 SD above background) |
|
|
Dynamic Range |
18.2pg/ml – 25ng/ml |
|
|
Minimal Detectable Dose (MDD) |
4.5pg/ml (2 SD above background) |
|
|
Assay Time |
70 minutes |
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Sample Type |
Cell culture supernatants |
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SD=Standard Deviation
Quantificação Sensível da Liberação de IL-4 em Baixo Nível
When treated with a Cell Stimulation Cocktail (CSC), T helper type 2 (Th2) cells and human PBMCs (PBMC) each release low levels of IL-4. Following 24 hours of CSC treatment, Lumit® IL-4 Immunoassay reagents were added directly to cell wells to measure IL-4 release.
Multiplexação no Mesmo Poço com Ensaios de Viabilidade e Citotoxicidade
Human T helper type 2 (Th2) cells were plated in 96-well plate format, primed overnight with hIL-2 and treated with increasing concentration of cell stimulation cocktail (CSC) for 24 hours. Following treatment, CellTox™ Green Cytotoxicity Assay and the Lumit® IL-4 (Human) Immunoassay reagents were added to determine cytotoxicity and IL-4 release. CellTiter-Glo® Cell Viability Assay reagent was added to determine cell viability. A dose-dependent increase in IL-4 was observed, with some degree of increased cytotoxicity and decreased cell viability.
Explore Outros Ensaios de Detecção de Citocinas
Oferecemos um crescente portfólio de ensaios para detecção de citocinas em amostras de cultura de células.
Não’ encontra seu alvo na lista?
Por favor, informe-se sobre o acesso antecipado a novos ensaios em desenvolvimento.
Para uma empresa de biotecnologia que entende que o tempo tem um valor significativo, os imunoensaios Lumit™ são uma forma essencial de coletar dados confiáveis rapidamente para que possamos chegar a conclusões e tomar decisões mais rapidamente.
Jonathan Chow, Cientista Principal, Corner Therapeutics
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Código | Tamanho | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml | |
Human IL-4 Standard |
W125A | 1 × 25μl | 10μg/ml |
Anti-hIL-4 mAb-SmBiT, 500X |
W126A | 1 × 30μl | |
Anti-hIL-4 mAb-LgBiT, 500X |
W127A | 1 × 30μl |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Código | Tamanho | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml | |
Human IL-4 Standard |
W125A | 1 × 25μl | 10μg/ml |
Anti-hIL-4 mAb-SmBiT, 500X |
W126A | 5 × 30μl | |
Anti-hIL-4 mAb-LgBiT, 500X |
W127A | 5 × 30μl |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Código | Tamanho | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml | |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml | |
Human IL-4 Standard |
W125A | 1 × 25μl | 10μg/ml |
Anti-hIL-4 mAb-SmBiT, 500X |
W126B | 1 × 300μl | |
Anti-hIL-4 mAb-LgBiT, 500X |
W127B | 1 × 300μl |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
What's in the box?
| Item | Código | Tamanho | Concentration |
|---|---|---|---|
Human IL-4 Standard |
W125A | 1 × 25μl | 10μg/ml |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
Resources
Outros recursos
- Rapid and Sensitive Determination of Cytokine Release from Cells Without Sample Transfer
- Blog Article: Understanding Inflammation: A Faster, Easier Way to Detect Cytokines in Cells
- Blog Article: The Cytokine Storm: Why Some COVID-19 Cases are More Severe
- White Paper: Lumit® Immunoassays: A Rapid and Sensitive Method for Analyte Detection
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