CDK5R1 Expression Vector
Regulatory Vector for NanoBRET® Kinase Target Engagement
- Transfection-ready DNA to express full-length CDK5R1 regulatory protein
- Use with select kinase-NanoLuc® fusion vectors and NanoBRET® TE Intracellular Kinase Assay
Catalog Number:
Size
Catalog Number: NV2821
Express Regulatory Proteins in Mammalian Cells
The CDK5R1 Expression Vector contains the coding region of the human CDK5R1 regulatory protein. The expression vectors are designed for use with kinase-NanoLuc® Fusion Vectors that contain the coding region of a kinase-NanoLuc® (or NanoLuc®-kinase) fusion protein. The expression vector and kinase-NanoLuc® Fusion Vector are cotransfected into cells prior to analysis using the appropriate NanoBRET® Target Engagement Intracellular Kinase Assay. These regulatory protein expression vectors are supplied transfection-ready, with a CMV promoter that drives expression of the full-length regulatory protein.
For details on kinase-NanoLuc® fusion vectors that require co-expression with a regulatory protein expression vector, see the NanoBRET® Target Engagement Intracellular Kinase Assay Adherent Format Technical Manual, #TM598.
Vector Details:
- Gene name: CDK5R1
- Sequence
- Recommended Assay: NanoBRET® TE Intracellular Kinase Assay, Adherent Format
NanoBRET® Target Engagement (TE) Intracellular Kinase Assays
The NanoBRET® TE Intracellular Kinase Assays can analyze the affinity of test compounds by competitive displacement of a fluorescent NanoBRET® Tracer reversibly bound to a NanoLuc®-kinase fusion protein in cells. Introduction of competing test compounds results in a dose-dependent decrease in the NanoBRET® signal for estimating intracellular affinity against the target protein. The NanoBRET® Tracer used is determined by your choice of NanoLuc® kinase fusion vector. The Kinase Vector Selection table shows which NanoBRET® Tracer assay is correct for each kinase fusion vector.
Protocols
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Specifications
Catalog Number:
What's in the box?
| Item | Código | Tamanho | Concentration |
|---|---|---|---|
CDK5R1 Expression Vector |
NV282A | 3 × 20μg | 1μg/μl |
SDS
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Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
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