pGEM®-5Zf(+) Vector
Standard Cloning Vector and Template for In vitro Transcription
- Blue/white screening to easily identify recombinant clones
- Multiple cloning site provides a convenient selection of restriction sites for cloning
Catalog Number:
Size
Catalog Number: P2241
The pGEM®-5Zf(+) Vector serves as a standard cloning vector and as a template for in vitro transcription. This vector contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be identified directly by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region contains unique restriction sites for ApaI, AatII, SphI, NcoI, SacII, EcoRV, SpeI, NotI, PstI, SalI, NdeI, SacI, BstXI and NsiI.
Reference
- Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
pGEM®-5Zf(+) Vector GenBank® Accession Number X65308 and vector sequence text file.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Código | Tamanho |
|---|---|---|
|
pGEM®-5Zf(+) Vector |
P224A | 1 × 20μg |
SDS
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Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
Resources
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