RQ1 RNase-Free DNase
Degrades Single-Stranded or Double-Stranded DNA
- Produces 3´ hydroxyl oligonucleotides during degradation
- Preparation qualified for use in applications where maintaining the integrity of RNA is critical
- Intended for Laboratory Use (GPR) in the United States. Outside of the United States, this product is intended for research use only unless otherwise stated
Catalog Number:
Size
Catalog Number: M6101
RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides. The enzyme is provided with 10X Reaction Buffer (400mM Tris-HCl [pH 8.0 at 25°C], 100mM MgSO4, 10mM CaCl2) and Stop Buffer (20mM EGTA [pH 8.0 at 25°C])
Applications
- Preparation of DNA-free RNA.
- Degradation of DNA from RNA transcription systems.
- Nick translation of DNA.
- Studying DNA:protein interactions by DNase I footprinting.
Storage Buffer: 10mM HEPES (pH 7.5 at 25°C), 50% (v/v) glycerol, 10mM CaCl2, 10mM MgCl2.
Source: Bovine pancreas.
QC Tests: Activity, RNase.
Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1μg of DNA in 10 minutes at 37°C in 50μl of a buffer containing 40mM Tris-HCl (pH 7.9 at 25°C), 10mM NaCl, 6mM MgCl2, 10mM CaCl2.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Código | Tamanho | Available Separately |
|---|---|---|---|
|
RQ1 DNase 10X Reaction Buffer |
M198A | 1 × 1ml | |
|
RQ1 DNase Stop Solution |
M199A | 1 × 1ml | |
|
RQ1 RNase-Free DNase |
M610A | 1 × 1,000u | View Product |
SDS
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Restrições de uso
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Condições de armazenamento
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