SalI
- Capable of digesting DNA in 15 minutes or less
- Active and capable of digestion directly in GoTaq® Green Master Mix
- Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
- Genome qualified to ensure optimal performance in genomic analysis applications
Catalog Number:
Choose a concentration
Size
Catalog Number: R6051
Catalog Number: R6055
Cut Site:
G▼TCGA C
C AGCT▲G
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Usage Notes:
- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
- Ends generated by SalI can be directly ligated to ends generated by XhoI. Neither recognition site is regenerated in the ligation product.
Incubation Conditions: Buffer D. 37°C.
Source: Streptomyces albus G.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | MULTI-CORE™ |
|---|---|---|---|---|
| <10% | 10–25% | 25–50% | 100% | <10% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 2 | 3 | 0 | 1 | 1 | 1 |
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Código | Tamanho | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer D 10X Buffer |
R004A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
SalI |
R605A | 1 × 2,000u |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
What's in the box?
| Item | Código | Tamanho | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer D 10X Buffer |
R004A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396E | 1 × 750μl | 10mg/ml | View Product |
|
SalI |
R605H | 1 × 10,000u |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
Resources
Outros recursos
Related Products
Produtos semelhantes
Frequentemente usado com
LigaFast™ Rapid DNA Ligation System
Efficiently ligates DNA inserts into plasmid vectors in just 5–15 minutes at room temperature.
M8221, M8225
Blue/Orange Loading Dye, 6X
Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.
G1881
Agarose, LE, Analytical Grade
Reagent for electrophoretic separation of nucleic acids.
V3121, V3125
Ethidium Bromide Solution, Molecular Grade
Fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients.
H5041
