Lumit® FcRn Binding Immunoassay

The Lumit® FcRn Binding Immunoassay is a novel homogeneous (no-wash) competition assay to measure the interaction between human FcRn and Fc proteins, including antibodies. In the assay, a human IgG1 labeled with LgBiT (Tracer-LgBiT) is used as the tracer. A C-terminal biotinylated human FcRn bound to Streptavidin-SmBiT (hFcRn-Biotin-SA-SmBiT) is used as the target. In the absence of an antibody analyte sample, Tracer-LgBiT binds to the hFcRn-SmBiT target, resulting in maximum luminescence signal. In analyte samples, unlabeled IgG will compete with Tracer-LgBiT for binding to the FcRn target, resulting in a concentration-dependent decrease in luminescent signal.

FcRn Function
The neonatal Fc receptor (FcRn) is expressed in the endosomal compartments of a variety of cell types, including vascular endothelium and antigen-presenting cells (APCs). FcRn binds to the Fc region of immunoglobulin G (IgG) antibodies at acidic pH within endosomes. In utero, FcRn acts to transfer maternal IgG to the developing fetus. In adults, it is involved in recycling of IgG and albumin. Recycling by FcRn is the primary reason for the long half-life (several weeks) of IgG and albumin in serum. Furthermore, a critical factor for the success of therapeutic antibodies is their extended half-life, which contributes to better efficacy and long dosing schedule. Therefore, the FcRn-IgG interaction is a key parameter to optimize and track throughout the antibody drug development process.

Note: The total number of assays per kit will vary based on final well volume.

For standard well volumes:

• 100 assay kits: applicable for 100 assays in 96-well plates or 400 assays in 384-well plates.
• 1,000 assay kits: applicable for 1,000 assays in 96-well plates or 4,000 assays in 384-well plates.