Promoterless Renilla Luciferase Basic Vectors
Use to Clone in Putative Promoter Elements or as a Control Vector in DLR Assays
- Designed for cloning a putative promoter element for investigating gene transcription control regions
- Use as a promoterless control vector in a dual-reporter assay
Catalog Number:
Choose a luciferase gene
Size
Catalog Number: E6881
The pGL4.70[hRluc] Vector is designed primarily to accept a putative promoter element for investigation of important regions controlling gene transcription. Alternatively, this vector may be used as a promoterless control vector in a dual-reporter system with a firefly luciferase vector serving as the experimental vector. The promoterless vector is available with an hRluc engineered Renilla luciferase gene. The inserts cloned into this vector can easily be transferred using the multiple cloning site and a unique SfiI transfer scheme.
Applications
- Transcription regulation.
- Virus-cell interactions.
- Compound screening.
- Post-translational modifications.
- Promoter analysis.
How the Engineered <i>Renilla</i> Luciferase Genes Work
The hRluc gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization.
About the pGL4 Reporter Vectors
The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Renilla luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.
pGL4.70[hRluc] Vector GenBank® Accession Number AY738226 and vector sequence text file.
Specifications
Catalog Number:
What's in the box?
| Item | Código | Tamanho |
|---|---|---|
|
pGL4.70[hRluc] Vector |
E688A | 1 × 20μg |
SDS
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Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 7,906,282 and European Pat. No. 1341808.
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