IdeS Protease and IdeZ Protease
Antibody Fragmentation and Characterization, Simplified
- Highly specific IgG cleavage
- Rapid generation of highly pure fragments
- Frozen IdeS Protease and Frozen IdeZ Protease are no longer available
Catalog Number:
Choose a Protease
Size
Catalog Number: V7511
Catalog Number: V7515
Catalog Number: V8341
Catalog Number: V8345
IgG-Digesting Enzymes for Therapeutic Antibody Characterization
IdeS and IdeZ Proteases are immunoglobulin G (IgG)-degrading enzymes that are valuable tools for the characterization of therapeutic antibodies, Fc fusion proteins and antibody-drug conjugates.
IdeS and IdeZ Proteases are fast and easy-to-use; complete digestion can be achieved in 30 minutes without optimization. Cleavage is highly reproducible and specific at a single site below the hinge region.
Both IdeS and IdeZ Proteases effectively cleave human IgG1, IgG2, IgG3 and IgG4, monkey, sheep, rabbit, humanized and chimeric IgGs as well as Fc-fusion proteins. However, only IdeZ Protease cleaves mouse IgG2a and IgG3.
Cleavage Specificity of IdeS and IdeZ Proteases
IdeS and IdeZ Proteases are immunoglobulin G (IgG)-degrading proteases that cleave with high specificity.
IdeS Protease is derived from Streptococcus pyogenes. It is an engineered, recombinant protease overexpressed in E. coli. IdeS Protease cleaves IgG at a single site below the hinge region, yielding F(ab')2 and Fc fragments as shown here.
IdeZ Protease is derived from Streptococcus equi subspecies zooepidemicus. It is also an engineered, recombinant protease overexpressed in E. coli. Like IdeS, IdeZ Protease specifically cleaves IgG molecules below the hinge region, yielding F(ab')2 and Fc fragments. However, IdeZ Protease has significantly improved activity against mouse IgG2a and IgG3 subclasses compared to IdeS Protease.
Core and Lower Hinge Sequence of Human and Mouse IgG Subclasses
| Subclass | Hinge/CH2 Sequence | IdeS Activity | IdeZ Activity |
|---|---|---|---|
|
Human
|
|
|
|
|
IgG1
|
CPPCPAPELLGGPSVF
|
+++
|
+++
|
|
IgG2
|
CPPCPAPP_VAGPSVF
|
+++
|
+++
|
|
IgG3
|
CPRCPAPELLGGPSVF
|
+++
|
+++
|
|
IgG4
|
AHHAQAPEFLGGPSVF
|
+++
|
+++
|
| Subclass | Hinge/CH2 Sequence | IdeS Activity | IdeZ Activity |
|---|---|---|---|
|
Mouse
|
|
|
|
|
IgG1
|
PCICTVPEV_SSVF
|
–
|
–
|
|
IgG2a
|
CPPCAAPNLLGGPSVF
|
+
|
+++
|
|
IgG2b
|
CHKCPAPNLEGGPSVF
|
–
|
–
|
|
IgG3
|
GSSCPAGNILGGPSVF
|
+
|
+++
|
Preparation of F(ab′)2 Fragments using IdeS Protease and Magne™ Protein A Beads
Panel A. Generation of F(ab′)2 fragments using IdeS Protease and Magne™ Protein A Beads.
Panel B. Gels demonstrating separation of F(ab′)2 and Fc fragments from three antibodies. Fc fragments were captured using Magne™ Protein A beads.
Specifications
Catalog Number:
What's in the box?
| Item | Código | Tamanho |
|---|---|---|
IdeS Protease |
V751A | 1 × 5,000 units |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
What's in the box?
| Item | Código | Tamanho |
|---|---|---|
IdeS Protease |
V751A | 5 × 5,000 units |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
What's in the box?
| Item | Código | Tamanho |
|---|---|---|
IdeZ Protease |
V834A | 1 × 5,000 units |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
What's in the box?
| Item | Código | Tamanho |
|---|---|---|
IdeZ Protease |
V834A | 5 × 5,000 units |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
Resources
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