PureYield™ Plasmid Maxiprep System
Up to 1mg of Transfection-Ready Plasmid from 250ml Cultures
- One-hour, vacuum-based protocol
- Highly pure, concentrated plasmid DNA
- No tedious, gravity-drip columns or post-elution alcohol precipitation
Catalog Number:
Size
Catalog Number: A2392
Catalog Number: A2393
The PureYield™ Plasmid Maxiprep System purifies up to 1mg of transfection-quality plasmid DNA from 250ml bacterial cultures in under an hour. The system is designed for use with a vacuum source and vacuum manifold, greatly reducing the time spent on purification compared to silica resin or other membrane-based methods.
PureYield™ Plasmid Maxipreps include a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants generates highly pure DNA with improved performance in sensitive applications such as eukaryotic transfection, in vitro transcription and transcription/translation applications. Purification is achieved without isopropanol precipitation of purified plasmid DNA.
Advantages of the PureYield™ Plasmid Maxipreps System:
- Simplifies purification of multiple samples at one time.
- Eliminates tedious, gravity-drip columns and post-elution alcohol precipitation.
- Purifies large amounts of plasmid DNA, exceeding the capabilities of other maxiprep systems.
This product requires the use of a vacuum pump and vacuum manifold.
Protocols
Complete Protocol
Protocolos rápidos
Specifications
Catalog Number:
What's in the box?
| Item | Código | Tamanho | Available Separately |
|---|---|---|---|
|
Neutralization Solution (NSB) |
A148H | 1 × 130ml | View Product |
|
PureYield™ Clearing Columns |
A246A | 2 × 5 each | |
|
PureYield™ Maxi Binding Columns |
A248A | 2 × 5 each | |
|
Endotoxin Removal Wash |
A251E | 1 × 36.5ml | |
|
Column Wash |
A252D | 1 × 82ml | |
|
Cell Resuspension Solution (CRA) |
A711Q | 1 × 125ml | View Product |
|
Cell Lysis Solution (CLA) |
A712Q | 1 × 125ml | View Product |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
SDS
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Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
What's in the box?
| Item | Código | Tamanho | Available Separately |
|---|---|---|---|
|
Neutralization Solution (NSB) |
A148J | 1 × 315ml | View Product |
|
PureYield™ Clearing Columns |
A246A | 5 × 5 each | |
|
PureYield™ Maxi Binding Columns |
A248A | 5 × 5 each | |
|
Endotoxin Removal Wash |
A251C | 1 × 85.3ml | |
|
Column Wash |
A252C | 1 × 210ml | |
|
Cell Resuspension Solution (CRA) |
A7115 | 1 × 315ml | |
|
Cell Lysis Solution (CLA) |
A7125 | 1 × 315ml | |
|
Nuclease-Free Water |
P119C | 2 × 25ml | View Product |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
Resources
No related resources available
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