qPCR Kits: GoTaq® qPCR and RT-qPCR Systems
Convenient Master Mixes for Real-Time qPCR and RT-qPCR
- Ready-to-use mix of optimized components for dye-based qPCR or RT-qPCR
- Compatible with both fast and standard cycling methods
- Resistant to a wide range of PCR inhibitors
Catalog Number:
Choose a system
Size
Catalog Number: A6001
Catalog Number: A6002
Catalog Number: A6020
Catalog Number: A6010
Simple, Sensitive, Dye-Based qPCR and RT-qPCR
The GoTaq® qPCR and RT-qPCR Systems are ready-to-use, 2X master mixes containing BRYT Green® Dye, a fluorescent DNA binding dye with minimal PCR inhibition, providing maximum amplification efficiency and greater fluorescence enhancement than SYBR® Green I.
Rapid hot-start activation and processive enzymes make the GoTaq® Systems compatible with most real-time PCR instruments using standard or fast cycling. The 1-Step and 2-Step RT-qPCR Systems also include GoScript™ Reverse Transcriptase, to enable efficient synthesis of first-strand cDNA in preparation for PCR.
Choose qPCR or RT-qPCR Master Mix Formats
Sensitivity and Specificity for qPCR
The GoTaq® qPCR Master Mix is a ready-to-use 2X master mix for real-time quantitative PCR. Combining GoTaq® Hot Start Polymerase, optimized buffer and the BRYT Green® Dye, the GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad-range detection. These qualities result in increased reliability, reproducibility and sensitivity for quantitative, real-time PCR.
Designed for use with any instrument capable of reading SYBR® Green I or FAM™ Dye, the GoTaq® qPCR Master Mix offers:
- Sensitive detection for earlier quantitation of low- and high-copy targets.
- Room-temperature setup,making it suitable for automation and high-throughput detection.
- Compatibility with both fast and standard qPCR cycling methods.
| Cat.# | Size | 10µl Reactions | 20µl Reactions |
|---|---|---|---|
| A6001 | 5ml | 1,000 reactions | 500 reactions |
| A6002 | 25ml | 5,000 reactions | 2,500 reactions |
Reverse Transcription and qPCR in One Reaction
The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step real-time RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single reaction well. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.
Other features provided by the 1-Step System include:
- Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range. Detects down to femtogram (fg) amounts of input RNA.
- Exceptional room-temperature setup, allowing easy automation and high-throughput detection.
| Cat.# | Size | 10µl Reactions | 20µl Reactions |
|---|---|---|---|
| A6020 | 5ml | 1,000 reactions | 500 reactions |
Two-Step Reverse Transcription and qPCR
The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.
The GoTaq® qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.
The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.
| Cat.# | Size | 10µl Reactions | 20µl Reactions |
|---|---|---|---|
| A6010 | 5ml | 50 × 20µl RT reactions and 1,000 × 10µl qPCR reactions | 50 × 20µl RT reactions and 500 × 20µl qPCR reactions |
Performance Tested in qPCR
Suitable for Different Organisms and Sample Types
DNA/RNA isolated from the following organisms and sample types has been used with GoTaq® qPCR Systems. DNA/RNA from organisms and sample types not listed may also be compatible—please enquire with Promega technical support for additional compatibility.
Organism Types:
- Animal
- Bacteria
- Fungi
- Human
- Parasites
- Viruses
- Yeast
Sample Types:
- Blood
- Cells
- Feces
- FFPE
- Food
- Human Cerebrospinal Fluid (CSF)
- Plants
- Plasma
- Saliva
- Serum
- Sputum
- Swabs
- Tissue
- Urine
Compatible with these Instruments
For further details on instrument compatibility, please reference the appropriate Technical Manual. Instruments not listed may also be compatible—please enquire with Promega technical support for additional compatibility.
- Analytik Jena qTOWERiris PCR thermal cycler
- Applied Biosystems ABI PRISM® 7000 and 7700 Sequence Detection System
- Applied Biosystems 7300 and 7900HT Real-Time PCR System
- Applied Biosystems GeneAmp® 5700 Thermal Cycler
- Applied Biosystems StepOne™ and StepOnePlus™ Real-Time PCR Systems
- Applied Biosystems 7500 and 7500 FAST Real-Time PCR System
- Applied Biosystems ViiA® 7 Real-Time PCR System
- Applied Biosystems QuantStudio® Real Time PCR Systems
- Bio-Rad CFX96 Real-Time PCR Detection System
- Bio-Rad DNA Engine Opticon® and Opticon® 2 Real Time PCR Detection Systems
- Bio-Rad iCycler® iQ™ and iQ™ 5 Real-Time PCR Detection System
- Bio-Rad/MJ Research Chromo4™ Real-Time Detector
- Bio-Rad MyiQ™ Real-Time PCR Detection System
- Cepheid SmartCycler® System
- Corbett Rotor-Gene™ 3000 and 6000 Real-Time Rotary Analyzer
- Eppendorf Mastercycler® ep realplex Real-Time PCR System
- Roche LightCycler® 480 Real-Time PCR System
- Stratagene Mx3000P® and Mx3005P® Real-Time PCR Systems
- Stratagene Mx4000® Multiplex Quantitative PCR System
Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
Protocols
Complete Protocol
Protocolos rápidos
Specifications
Catalog Number:
What's in the box?
| Item | Código | Tamanho | Available Separately |
|---|---|---|---|
|
GoTaq® qPCR Master Mix, 2X |
A600A | 5 × 1ml | View Product |
|
CXR Reference Dye |
C541A | 1 × 100μl | View Product |
|
Nuclease-Free Water |
P119E | 2 × 13ml | View Product |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
What's in the box?
| Item | Código | Tamanho | Available Separately |
|---|---|---|---|
|
GoTaq® qPCR Master Mix, 2X |
A600A | 25 × 1ml | View Product |
|
CXR Reference Dye |
C541A | 5 × 100μl | View Product |
|
Nuclease-Free Water |
P119E | 10 × 13ml | View Product |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
What's in the box?
| Item | Código | Tamanho | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 1 × 750μl | 25mM | View Product |
|
GoTaq® qPCR Master Mix, 2X |
A600A | 5 × 1ml | View Product | |
|
Nuclease-Free Water |
P119E | 2 × 13ml | View Product | |
|
CXR Reference Dye |
C541B | 1 × 200μl | 30μM | View Product |
|
GoScript™ RT Mix for 1-Step RT-qPCR |
M700A | 1 × 225μl | 50X |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
What's in the box?
| Item | Código | Tamanho | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 1 × 750μl | 25mM | View Product |
|
GoScript™ 5X Reaction Buffer |
A500B | 1 × 300μl | View Product | |
|
GoScript™ Reverse Transcriptase |
A501B | 1 × 50μl | ||
|
GoTaq® qPCR Master Mix, 2X |
A600A | 5 × 1ml | View Product | |
|
Oligo(dT)15 Primer |
C110B | 1 × 50μg | 500μg/ml | View Product |
|
PCR Nucleotide Mix |
C114G | 1 × 200μl | 10mM | View Product |
|
Random Primers |
C118B | 1 × 50μg | View Product | |
|
CXR Reference Dye |
C541A | 1 × 100μl | View Product | |
|
Recombinant RNasin® Ribonuclease Inhibitor |
N251A | 1 × 2,500u | 40u/μl | View Product |
|
Nuclease-Free Water |
P119E | 2 × 13ml | View Product |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
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