TnT® SP6 High-Yield Wheat Germ Protein Expression System

The TnT® SP6 High-Yield Wheat Germ Protein Expression System contains optimized wheat germ extract in a single-tube, coupled transcription/translation system that allows you to express proteins in only two hours from plasmid or PCR-generated templates. Protein is synthesized in concentrations in the range of 10–100μg/ml and can be used in multiple applications including high-throughput analysis.

All components necessary for transcription/translation are provided in the extract, with the exception of the plasmid DNA or PCR template. Optional protein-labeling reagents must also be supplied by the user.

For more information, see the Protocols & Applications Guide.

For custom wheat germ extract (depleted amino acids), order Cat.# X808X.

Applications

• Protein:protein interactions
• Protein:nucleic acid interactions
• Protein:ligand interactions
• Enzyme assays
• Protein expression analysis

References

1. Falah, N. et al. (2012) J. Virol. 86, 691–704.
2. Stempin, C.C. et al. (2011) J. Biol. Chem. 286, 37147–57.
3. Liu, W. et al. (2011) Plant Cell. 23, 3853–65.
4. Shi, X. et al. (2011) J. Biol. Chem. 286, 33401–8.
5. Zhao, L. et al. (2010) J. Struct. Funct. Genomics 11, 201–9.
6. Zhao, K.Q., Hurst, R., Slater, M.R. and Bulleit, R.F. (2007) J. Struct. Funct. Genomics 8, 199–208.