HaloTag® Mammalian Protein Purification System
Recover Highly Pure Recombinant Protein from Mammalian Cell Cultures, Even at Low Expression Levels
- HaloLink™ Resin covalently binds >7mg/ml of HaloTag® fusion protein (10X more capacity than FLAG®)
- Easy to scale up or down to obtain milligram + quantities of protein
- Choose from purification, purification + detection or sample purification + detection kits
Catalog Number:
Choose Mammalian Purification Kit
Size
Catalog Number: G6790
Catalog Number: G6795
Streamline Your Protein Purification Process
The HaloTag® Mammalian Protein Purification System (Cat.# G6790) is an optimized kit for purification of HaloTag® fusion proteins from mammalian cell culture lysates. HaloTag® fusion proteins form a highly specific and covalent bond with the HaloLink™ Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink™ Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag® Mammalian Protein Detection and Purification System (Cat.# G6795 or G6799) also includes HaloTag® TMRDirect™ Ligand. The simple-to-use fluorescent detection of the HaloTag® fusion facilitates rapid optimization of expression and purification conditions.
Streamlined purification process leads to higher purity and recovery of recombinant proteins from cultured cells.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Código | Tamanho | Concentration | Available Separately |
|---|---|---|---|---|
|
HaloLink™ Resin |
G1913 | 1 × 2.5ml | ||
|
Protease Inhibitor Cocktail, 50X |
G652A | 2 × 1ml resuspended | View Product | |
|
HaloTEV Protease |
G660A | 1 × 200μl | 5u/μl | View Product |
|
Spin Column |
V995A | 1 × 25 each |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
See Protocol for detailed storage recommendations.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box?
| Item | Código | Tamanho | Concentration | Available Separately |
|---|---|---|---|---|
|
HaloLink™ Resin |
G1913 | 1 × 2.5ml | ||
|
HaloTag® TMRDirect™ Ligand |
G299A | 1 × 30μl | View Product | |
|
Protease Inhibitor Cocktail, 50X |
G652A | 2 × 1ml resuspended | View Product | |
|
HaloTEV Protease |
G660A | 1 × 200μl | 5u/μl | View Product |
|
Spin Column |
V995A | 1 × 25 each |
SDS
Search for SDSCertificado de Análise
Restrições de uso
For Research Use Only. Not for Use in Diagnostic Procedures.Condições de armazenamento
See Protocol for detailed storage recommendations.
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
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