jb1001-bio-glo-nb-tck-luciferase-assay-system-10ml jb1002-bio-glo-nb-tck-luciferase-assay-system-100ml jb1003-bio-glo-nb-tck-luciferase-assay-system-10x100ml

Bright, Long-Lasting Luciferase Reagent for Target Cell Killing Assays

  • Detection reagent designed for HiBiT target cell killing bioassays
  • Simple add-mix-read protocol for rapid bioassay results
  • Quality controlled and tested for optimal reliability

TCK Luciferase Assay Systems

  Catalog No. Product Name   Size Price Qty  
JB1001 Bio-Glo-NB™ TCK Luciferase Assay System View Specifications 10ml     Solicitar cotação
JB1002 Bio-Glo-NB™ TCK Luciferase Assay System View Specifications 100ml     Solicitar cotação
JB1003 Bio-Glo-NB™ TCK Luciferase Assay System View Specifications 10 × 100ml     Solicitar cotação

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Overview
Protocols
Specifications
Resources
Related Products

Quantify Targeted Cell Killing with Confidence

The Bio-Glo-NB™ Luciferase Assay Systems provide a highly sensitive, robust and homogeneous reagent for the detection of cell killing in Promega TCK Bioassays. Bio-Glo-NB™ Assay Reagents contain a cell-impermeable LgBiT that complements a HiBiT-tagged protein of interest in the extracellular media, resulting in a bright, luminescent NanoBiT® luciferase. Bio-Glo-NB™ Luciferase Assay Systems are functionally tested for performance in the Target Cell Killing Bioassays and intended for use with those products.

The Bio-Glo-NB™ Luciferase Assay Systems provide a highly sensitive, robust and homogeneous reagent for the detection of cell killing in Promega TCK Bioassays.

 

Created with BioRender.com

Simple Add-Mix-Read Protocol

Depiction of the Bio-Glo-NB™ TCK Luciferase Assay protocol workflow.

HiBiT TCK Bioassay Luminescence Reporter Enables Time Course Measurements

Line graph illustrating that Raji (HT-HiBiT) TCK Bioassays are amenable for use with CAR-T effector cells.
Line graph illustrating that H929 Cells (HT-HiBiT) are amenable to use in HiBiT TCK Bioassays using CAR-T effector cells.

Bio-Glo-NB™ TCK Reagent is Optimized for Use to Include Extended Co-culture Applications. Bio-Glo-NB™ TCK Reagent is optimized for batch and continuous-process handling; the extended bright light output offers high sensitivity, especially for extended incubations. HiBiT target cells were incubated with the respective CAR-T cells at the indicated time points before Bio-Glo-NB™ TCK Reagent was added. Panel A. Raji (HiBiT) cells. Panel B. H929 (HiBiT) cells.

Specifications

Catalog Number:

What's in the box?

Item Código Tamanho

Bio-Glo-NB™ TCK Luciferase Assay Substrate

JB100A 1 × 0.2ml

Bio-Glo-NB™ TCK Luciferase Assay Buffer

JB101A 1 × 10ml

Bio-Glo-NB™ TCK LgBiT Protein

JB102A 1 × 0.1ml

Certificado de Análise

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Restrições de uso

For Research Use Only. Not for Use in Diagnostic Procedures.

Condições de armazenamento

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What's in the box?

Item Código Tamanho

Bio-Glo-NB™ TCK Luciferase Substrate

JB100B 2 × 1ml

Bio-Glo-NB™ TCK Luciferase Assay Buffer

JB101B 1 × 100ml

Bio-Glo-NB™ TCK LgBiT Protein

JB102B 1 × 1ml

Certificado de Análise

Search by lot number

Restrições de uso

For Research Use Only. Not for Use in Diagnostic Procedures.

Condições de armazenamento

BB

What's in the box?

Item Código Tamanho

Bio-Glo-NB™ TCK Luciferase Substrate

JB100C 5 × 4ml

Bio-Glo-NB™ TCK Luciferase Assay Buffer

JB101B 10 × 100ml

Bio-Glo-NB™ TCK LgBiT Protein

JB102B 10 × 1ml

Certificado de Análise

Search by lot number

Restrições de uso

For Research Use Only. Not for Use in Diagnostic Procedures.

Condições de armazenamento

BB