Product and Technology Guides
Learn more about basic and advanced methods for cell biology, nucleic acid analysis, protein analysis and basic research in these educational resources. Each guide provides an overview of the basic science behind the methods and techniques described, as well as links to products and other helpful resources.
Cell Biology
Introdução à Cultura de Células 3D
As culturas de células 3D estão sendo cada vez mais usadas para pesquisa básica e descoberta de medicamentos. Aqui você aprenderá o que são culturas de células 3D, por que e como elas estão sendo usadas e como validar ensaios que funcionam com modelos 3D.
ATP Assays
Learn how ATP assays work and how to choose the right assays for your needs.
Cell Line Authentication Resources
Misidentified cell lines can lead to lost resources, time and effort. Explore why cell line authentication is important and learn how to confirm the identity of your cells.
Escolhendo um Ensaio de Luciferase Repórter
Um guia comparativo para te ajudar a escolher o ensaio de luciferase para as suas necessidades.
Interpreting Multiplex Cell Health Data
The CellTox Green Cytotoxicity Assay can be used with other cell health assays to provide richer information about why and how cells die. This interactive guide teaches you how to interpret the results from these experiments.
Live-Cell Kinetic Assays for Time Course Analysis
Learn how live-cell kinetic assay kits allow you to repeatedly measure one sample well over time, allowing you to get better data while saving time and precious samples.
Assays for Biochemical Targets
This selector tool is designed to help you quickly find the right Promega assay for your protein target of interest.
How to Measure Cellular Metabolism
Learn how to measure cellular metabolism using fast, simple, bioluminescent assays. Explore methods for glycolysis, glutaminolysis, lipogenesis, and more.
Getting Started with Measuring Cellular Metabolism
A guide to choosing the right assay, setting up your experiment, and interpreting results with confidence.
Cell Imaging
An overview of methods used for imaging studies in cell biology.
Reporter Genes and their Applications
How to use bioluminescent reporter genes to study eukaryotic gene expression and cellular events.
Apoptosis
An introduction to apoptotic cell death, including methods to confirm apoptosis.
Guia de ensaio de viabilidade celular
Como escolher o ensaio de viabilidade celular correto para as suas necessidades.
Transfecção
Um guia para transfecção, incluindo protocolos e dicas de otimização.
Nucleic Acid Analysis
qPCR and RT-qPCR
A comprehensive introduction to qPCR and RT-qPCR methods.
Molecular Cloning Guide
A guide to the fundamentals of molecular cloning, including restriction digestion, DNA ligation, vector dephosphorylation, and bacterial transformation.
How to Use Restriction Enzymes: A Resource Guide
Restriction enzymes 101, including reference information and links to restriction digest tools.
Introduction to Sequencing
An in-depth overview of DNA sequencing, covering the principles, techniques, and applications of fragment analysis, Sanger sequencing, and next-generation sequencing (NGS), while also highlighting future innovations in genomics.
Purificação de DNA
Uma visão geral dos métodos de extração de DNA para necessidades de purificação manual e automatizada.
RNA Purification
An overview of RNA extraction with details for both manual and automated purification methods.
Epigenética
Uma introdução ao estudo das mudanças epigenéticas na expressão gênica.
Amplificação de PCR
Uma visão geral dos métodos de PCR, RT-PCR e qPCR.
Protein Analysis
Protease Digestion for Mass Spectrometry
A collection of protocols for in-gel and in-solution protein digestion with trypsin, trypsin/lys-c or alternative proteases.
Protein Expression
An introduction to in vitro transcription and translation methods.
Protein Purification Methods Guide
An overview of common methods for protein purification.
A Proteomics Product Guide
An overview of products used for protein expression, purification and analysis.