HEK293 LgBiT Cell Line and LgBiT Expression Vector Technical Manual
Instructions for Use of Product(s)
N2672, N2681
Literature # TM620
The HEK293 LgBiT Cell Line and LgBiT Expression Vector are reagents for intracellular expression of the LgBiT protein that complements HiBiT, an 11-amino-acid peptide tag, to produce luminescence in live cells in the presence of substrate. The LgBiT reagents can be paired with HiBiT protein fusions that are expressed transiently, stably or endogenously inserted by CRISPR-Cas9, and used for endpoint or kinetic real-time monitoring of HiBiT protein levels. In addition, these products can be multiplexed with cell-health assays and bioluminescent resonance energy transfer (BRET)-based applications, including NanoBRET™ protein:protein and target engagement assays.
Printed in USA 4/20.