Infectious Diseases
Infectious disease research covers a broad range of topics including microbial pathogenesis, epidemiology, immunology, and antimicrobial drug and vaccine development. Promega supplies many basic molecular biology reagents that are used extensively by researchers studying these disease processes. These products include genomic DNA, plasmid DNA and RNA extraction kits, sequencing reagents, cell health assays, cloning enzymes, and PCR systems.
In addition to these routine molecular tools, luminescence technologies such as firefly and NanoLuc® luciferase reporters and HiBiT protein tagging and detection system have found extensive application in the study of viral diseases. The small size of the Nanoluc® luciferase reporter and HiBiT tag make them particularly useful in small viral genomes, and the bright luminescence signal generated makes it possible to gain sensitivity in detecting viral activity in cells and organisms.
Viral Research, Testing and Assay Development
Infectious Disease Product Categories
Cell Health Assays
Assays to measure apoptosis, viability, cytotoxicity and inflammation.
Cloning and DNA Markers
Enzimas de restrição, vetores de clonagem, marcadores de peso molecular e células competentes.
Energy Metabolism
Ensaios bioluminescentes para medir a absorção de glicose, atividade de NAD(P)H, entre outros.
Immunoassays
Ensaios Lumit™ para imunodetecção rápida e bioluminescente, bem como anticorpos primários e secundários e reagentes para blotting.
Luciferase Assays
Ensaios e vectores de repetição, linhas celulares com expressão de luciferase e reagentes de transfecção.
Nucleic Acid Extraction
Kits adaptáveis que geram altos rendimentos a partir de diversos tipos de amostras e demandas.
PCR
Hot-start, PCR endpoint, qPCR e RT-PCR.
Protein Interactions
Ensaios de interação e de extração de proteínas com base em BRET, HaloTag e GST.
Sequencing
Produtos e soluções para modelos de entrada NGS de alta qualidade ou testes de confirmação.
Example Infectious Disease Applications
Virology
Luciferases are increasingly being used to study viral infectious diseases. These bioluminescence-based methods provide highly sensitive detection of specific cellular events such as viral entry, proliferation, and viral:host protein interactions.
Cellular Immune Response
Promega cell health and energy metabolism products are applied to study many aspects of the host response to pathogens, including inflammasome activation, host cell metabolic changes and host cell viability.
